LOCUS       sequence-280049-        2525 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0473
FEATURES             Location/Qualifiers
     source          1..2525
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccagca gggccagcag gggcagatgg caagtggaga cagcaatctc
       61 aacttcagga tggtcgcaga gatccagaac gtagagggtc agaacctgca ggagcaggtt
      121 tgccctgagc ccattttcag gttcttcaga gaaaacaagg tggagattgc aagtgcaata
      181 acaaggccat ttcctttcct tatgggcctc cgagaccgct ccttcatctc cgagcagatg
      241 tatgaacatt ttcaagaagc ttttagaaac ctggtcccag tgacaagagt gatgtattgt
      301 gtactcagtg aactggagaa gacatttggc tggtcacatc tggaagcatt gttcagcagg
      361 attaacctga tggcctatcc tgatttaaac gagatttaca gaagcttcca gaatgtatgc
      421 tatgaacact cacctctcca aatgaataat gtaaacgatt tagaagatag acccagatta
      481 ctaccatatg gtaaacaaga gaacagcaat gcctgtcatg aaatggatga tatagcagtg
      541 cctcaggaag ccttgagctc ctcgccaagg tgtgagccag gtttctcttc agagtcttgt
      601 gagcagttag ctctcccaaa ggctggtgga ggagatgctg aagatgcacc cagcctacta
      661 ccaggtgggg gagttctaga aagcaacggg atgatagatg cggcaaggac atacagcaca
      721 gcaccagggg agaaacaggg agaggaggaa ggcaggaaca gtcccagaaa aagaaaccaa
      781 gacaaggaga agtaccaaga gagtccagag ggaagagaca aagagacctt tgatctaaaa
      841 actccccaag tcactaatga aggagaacca gagaaggggc tctgtctact accaggtgaa
      901 ggagaagagg gcagtgatga ctgttcagaa atgtgtgatg gagaagagcc ccaggaagcc
      961 tctagctccc tagcaagatg tgggtcagag ggcagtgatg actgttcgga aatgtgtgat
     1021 ggagaagagc gccaggaagc ctctagctcc ctagcaagac gtgggtcagt gtctagtgaa
     1081 ctagaaaatc acccaatgaa tgaagaagga gaatcagaag agcttgcttc tagcctgcta
     1141 tatgataatg taccaggagc ggagcaatca gcatatgaaa atgagaagtg ttcctgtgtc
     1201 atgtgtttct cagaagaggt gccaggaagc ccagaagcaa ggacggaaag tgatcaagcg
     1261 tgtggcacaa tggatactgt ggatattgca aacaactcca ctttgggaaa acccaagagg
     1321 aaaagaagaa aaaagagggg gcatggctgg agcagaatga gaatgagaag gcaggaaaac
     1381 agccaacaaa atgataatag caaagccgac ggccaggtgg tctccagtga aaagaaggcg
     1441 aacgtgaatc tgaaagacct ttccaagatt agggggagaa agagaggcaa acctggaacc
     1501 cgcttcactc agagtgacag agctgcacag aaaagagtcc gatcaagagc ttcaagaaag
     1561 cacaaagatg aaactgtgga ttttaaggct cctttgcttc cagtgacctg tggtggggtg
     1621 aagggaattt tacataagaa gaaattgcag caaggaatct tggtgaagtg tatacagact
     1681 gaggatggaa aatggttcac ccccacggaa tttgaaatca aaggaggcca tgcaagatca
     1741 aagaactgga ggctgagtgt gcgctgtggc gggtggcccc tacgatggct gatggagaat
     1801 ggatttctgc ctgatcctcc aagaatacgt tacaggaaaa aaaagagaat actgaagtct
     1861 caaaacaata gctcagttga cccttgtatg agaaacctgg atgagtgtga ggtgtgccgg
     1921 gacggagggg agctgttctg ttgcgacact tgttcaagag tcttccatga ggactgtcac
     1981 atcccgcctg tggaagctga gaggaccccg tggaattgca tcttctgcag gatgaaggag
     2041 tctccgggaa gccaacagtg ttgtcaggaa tctgaggtcc tggagaggca gatgtgtcct
     2101 gaggaacagt tgaaatgtga gttcctcctc ttgaaagtct attgctgttc tgagagctcc
     2161 ttttttgcca agattccata ctattattat attagagagg cgtgtcaagg cctgaaggag
     2221 cccatgtggt tggataaaat caagaaaagg ctgaatgagc acggttaccc ccaagtggag
     2281 gggtttgtac aagacatgcg cctcatcttc cagaaccaca gggcctctta caagtacaag
     2341 gattttggcc aaatgggatt tagactggag gctgagtttg agaagaattt caaggaagtg
     2401 tttgctattc aggaaacaaa tgggaacaat tagtaatgaa ctagtccgta acttgaaagt
     2461 atttcgattt cttggcttta tatatcttgt ggaaaggacg attacagtct gctaccggtt
     2521 taaat
//
