LOCUS       sequence-280052-        2309 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0481
FEATURES             Location/Qualifiers
     source          1..2309
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagggcgc cggcggcgcg aacgacaaga aaaagataag ttctgaacgt
       61 cgaaaagaaa agtctcgaga tgcagccaga tctcggcgaa gtaaagaatc tgaagttttt
      121 tatgagcttg ctcatcagtt gccacttcca cataatgtga gttcgcatct tgataaggcc
      181 tctgtgatga ggcttaccat cagctatttg cgtgtgagga aacttctgga tgctggtgat
      241 ttggatattg aagatgacat gaaagcacag atgaattgct tttatttgaa agccttggat
      301 ggttttgtta tggttctcac agatgatggt gacatgattt acatttctga taatgtgaac
      361 aaatacatgg gattaactca gtttgaacta actggacaca gtgtgtttga ttttactcat
      421 ccatgtgacc atgaggaaat gagagaaatg cttacacaca gaaatggcct tgtgaaaaag
      481 ggtaaagaac aaaacacaca gcgaagcttt tttctcagaa tgaagtgtac cctaactagc
      541 cgaggaagaa ctatgaacat aaagtctgca acatggaagg tattgcactg cacaggccac
      601 attcacgtat atgataccaa cagtaaccaa cctcagtgtg ggtataagaa accacctatg
      661 acctgcttgg tgctgatttg tgaacccatt cctcacccat caaatattga aattccttta
      721 gatagcaaga ctttcctcag tcgacacagc ctggatatga aattttctta ttgtgatgaa
      781 agaattaccg aattgatggg atatgagcca gaagaacttt taggccgctc aatttatgaa
      841 tattatcatg ctttggactc tgatcatctg accaaaactc atcatgatat gtttactaaa
      901 ggacaagtca ccacaggaca gtacaggatg cttgccaaaa gaggtggata tgtctgggtt
      961 gaaactcaag caactgtcat atataacacc aagaattctc aaccacagtg cattgtatgt
     1021 gtgaattacg ttgtgagtgg tattattcag cacgacttga ttttctccct tcaacaaaca
     1081 gaatgtgtcc ttaaaccggt tgaatcttca gatatgaaaa tgactcagct attcaccaaa
     1141 gttgaatcag aagatacaag tagcctcttt gacaaactta agaaggaacc tgatgcttta
     1201 actttgctgg ccccagccgc tggagacaca atcatatctt tagattttgg cagcaacgac
     1261 acagaaactg atgaccagca acttgaggaa gtaccattat ataatgatgt aatgctcccc
     1321 tcacccaacg aaaaattaca gaatataaat ttggcaatgt ctccattacc caccgctgaa
     1381 acgccaaagc cacttcgaag tagtgctgac cctgcactca atcaagaagt tgcattaaaa
     1441 ttagaaccaa atccagagtc actggaactt tcttttacca tgccccagat tcaggatcag
     1501 acacctagtc cttccgatgg aagcactaga caaagttcac ctgagcctaa tagtcccagt
     1561 gaatattgtt tttatgtgga tagtgatatg gtcaatgaat tcaagttgga attggtagaa
     1621 aaactttttg ctgaagacac agaagcaaag aacccatttt ctactcagga cacagattta
     1681 gacttggaga tgttagctcc ctatatccca atggatgatg acttccagtt acgttccttc
     1741 gatcagttgt caccattaga aagcagttcc gcaagccctg aaagcgcaag tcctcaaagc
     1801 acagttacag tattccagca gactcaaata caagaaccta ctgctaatgc caccactacc
     1861 actgccacca ctgatgaatt aaaaacagtg acaaaagacc gtatggaaga cattaaaata
     1921 ttgattgcat ctccatctcc tacccacata cataaagaaa ctactagtgc cacatcatca
     1981 ccatatagag atactcaaag tcggacagcc tcaccaaaca gagcaggaaa aggagtcata
     2041 gaacagacag aaaaatctca tccaagaagc cctaacgtgt tatctgtcgc tttgagtcaa
     2101 agaactacag ttcctgagga agaactaaat ccaaagatac tagctttgca gaatgctcag
     2161 agaaagcgaa aaatggaaca tgatggttca ctttttcaag cagtaggaat tatttagtaa
     2221 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     2281 gacgagacgt ttgcaggtga ccgtgctat
//
