LOCUS       sequence-28006-e         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Novina CD, Cheriyath V, Roy AL
  TITLE     Regulation of TFII-I activity by phosphorylation.
  JOURNAL   J Biol Chem. 1998 Dec 11. 273(50):33443-8.
  PUBMED    9837922
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1998 Dec 11. 273(50):33443-8."
COMMENT     Sequence Label: pEBG-TFII-I Y248&249F
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..35
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             45..62
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccaccatggg cagcagccat catcatcatc atcacagcag cggcctggtg ccgcgcggca
       61 gccatatggc ccaagttgca atgtccaccc tccccgttga agatgaggag tcctcggaga
      121 gcaggatggt ggtgacattc ctcatgtcag ctctcgagtc catgtgtaaa gaactggcca
      181 agtccaaagc cgaagtggcc tgcattgcag tgtatgaaac agacgtgttt gtcgtcggaa
      241 ctgaaagagg acgtgctttt gtcaatacca gaaaggattt tcaaaaagat tttgtaaaat
      301 attgtgttga agaagaagaa aaagctgcag agatgcataa aatgaaatct acaacccagg
      361 caaatcggat gagtgtagat gctgtagaaa ttgaaacact cagaaaaaca gttgaggact
      421 atttctgctt ttgctatggg aaagctttag gcaaatccac agtggtacct gtaccatatg
      481 agaagatgct gcgagaccag tcggctgtgg tagtgcaggg gcttccggaa ggtgttgcct
      541 ttaaacaccc cgagaactat gatcttgcaa ccctgaaatg gattttggag aacaaagcag
      601 gaatttcatt catcattaag agaccttttt tagagccnaa gaagcatgta ggtggtcgtg
      661 tgatgg
//
