LOCUS       sequence-280070-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0529
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggcgcaga cggcatgtat gataaactaa ggatgttgaa cgggcagacc
       61 ggaagctggg gcacccgccc tggttggtat ccggggactt cggtgccagg gcaacctacg
      121 caagatggct gccagcaaca ggaaggaggg ggagagaata ccaactccat cagttccaac
      181 ggagaagatt cagatgaggc tcaaatgcga cttcagctga agcggaagct gcaaagaaat
      241 agaacatcct ttacccaaga gcaaattgag gccctggaga aagagtttga gagaacccat
      301 tatccagatg tgtttgcccg agaaagacta gcagccaaaa tagatctacc tgaagcaaga
      361 atacaggtat ggttttctaa tcgaagggcc aaatggagaa gagaagaaaa actgaggaat
      421 cagagaagac aggccagcaa cacacctagt catattccta tcagcagtag tttcagcacc
      481 agtgtctacc aaccaattcc acaacccacc acaccggttt cctccttcac atctggctcc
      541 atgttgggcc gaacagacac agccctcaca aacacctaca gcgctctgcc gcctatgccc
      601 agcttcacca tggcaaataa cctgcctatg caacccccag tccccagcca gacctcctca
      661 tactcctgca tgctgcccac cagcccttcg gtgaatgggc ggagttatga tacctacacc
      721 cccccacata tgcagacaca catgaacagt cagccaatgg gcacctcggg caccacttca
      781 acaggactca tttcccctgg tgtgtcagtt ccagttcaag ttcccggaag tgaacctgat
      841 atgtctcaat actggccaag attacagtag taatgaacta gtccgtaact tgaaagtatt
      901 tcgatttctt ggctttatat atcttgtgga aaggacgacc cggctattcc tacacgaacc
      961 ac
//
