LOCUS       sequence-280073-        1127 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0547
FEATURES             Location/Qualifiers
     source          1..1127
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagccagc cttcggggag gtgaaccagc tgggaggagt gttcgtgaac
       61 gggaggccgc tgcccaacgc catccggctt cgcatcgtgg aactggccca actgggcatc
      121 cgaccgtgtg acatcagccg ccagctacgg gtctcgcacg gctgcgtcag caagatcctg
      181 gcgcgataca acgagacggg ctcgatcttg ccaggagcca tcgggggcag caagccccgg
      241 gtcactaccc ccaccgtggt gaaacacatc cggacctaca agcagagaga ccccggcatc
      301 ttcgcctggg agatccggga ccgcctgctg gcggacggcg tgtgcgacaa gtacaatgtg
      361 ccctccgtga gctccatcag ccgcattctg cgcaacaaga tcggcaactt ggcccagcag
      421 ggtcattacg actcatacaa gcagcaccag ccgacgccgc agccagcgct gccctacaac
      481 cacatctact cgtaccccag ccctatcacg gcggcggccg ccaaggtgcc cacgccaccc
      541 ggggtgcctg ccatccccgg ttcggtggcc atgccgcgca cctggccctc ctcgcactcc
      601 gtcaccgaca tcctgggcat ccgctccatc accgaccaag tgagcgacag ctccccctac
      661 cacagcccca aggtggagga gtggagcagc ctgggccgca acaacttccc cgccgccgcc
      721 ccgcacgcgg tgaacgggtt ggagaaggga gccctggagc aggaagccaa gtacggtcag
      781 gcaccaaatg gtctcccagc tgtgggcagt tttgtgtcag catccagcat ggctccttac
      841 cctaccccag cccaagtgtc gccttacatg acctacagtg ctgctccttc tggttatgtt
      901 gctggacatg ggtggcaaca tgctgggggc acctcattgt ctccccacaa ctgtgacatt
      961 ccggcatcgc tggcgttcaa gggaatgcag gcagccagag aaggtagtca ttctgtcacg
     1021 gcttccgcgc tctagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1081 tatatatctt gtggaaagga cgaacctgga acttaaactt agaatga
//
