LOCUS       sequence-280088-        1436 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0577
FEATURES             Location/Qualifiers
     source          1..1436
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcctgcct caacccggtg ctctgggaaa ccaaggtttt gcagtctaaa
       61 gcaacagaag acttcttcag aaatagcatc caatcacctc tgtttactct tcagttgccg
      121 cacagcttgc accaaacatt gcaatcgtca acagcatcat tagttattcc tgctggtaga
      181 actcttccaa gttttaccac agcagaactg ggcacttcaa actccagtgc aaactttact
      241 tttcctggtt atcccattca tgtaccagca ggtgtgacac tacagactgt atctggtcac
      301 ctttataaag tcaatgtacc aattatggtg acagagactt ctggaagagc aggtattctt
      361 cagcatccaa ttcagcaagt atttcaacag cttggccagc cttcagtaat acaaactagt
      421 gttccacaat tgaatccatg gtctcttcaa gcaactactg aaaaatcaca gagaattgaa
      481 accgtgctac agcaacccgc aattctacct tctgggccag tagataggaa acacttagaa
      541 aatgccacca gtgatatact tgtatctcct ggaaatgagc ataaaatcgt gcctgaagct
      601 ttgttgtgtc atcaggaaag ttctcactat atcagtcttc caggtgttgt attttctcca
      661 caggtctctc aaacaaattc taatgtggag tcagtgctca gtggttcagc tagcatggct
      721 caaaatctgc atgatgagtc cctctccaca agccctcatg gggctctcca ccagcacgtg
      781 actgatattc agcttcatat tcttaaaaat aggatgtatg gatgtgattc tgtaaagcaa
      841 ccaagaaata tagaggaacc cagcaacata cctgtatcag agaaggattc taattctcag
      901 gtggatttaa gcattcgggt tactgatgat gatattggtg aaataattca agtagatgga
      961 agcggtgata catcttccaa tgaagaaata ggaagtacaa gagatgcaga tgagaatgaa
     1021 tttctaggga atattgacgg gggagatctg aaggtacctg aagaagaagc tgacagtatt
     1081 tcaaatgagg attcagccac aaacagtagt gataatgaag accctcaagt aaacattgta
     1141 gaagaggacc ctttaaattc tggagatgat gttagtgaac aggatgtgcc agacctgttt
     1201 gacacggata atgttattgt ctgtcagtat gataagattc atcgaagcaa gaacaaatgg
     1261 aaattctatt tgaaagatgg tgttatgtgt tttggaggga gagactatgt atttgcaaaa
     1321 gccattggtg atgcagagtg gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1381 tcttggcttt atatatcttg tggaaaggac gaggcagcac taacgcctat attctt
//
