LOCUS       sequence-280097-        2069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0589
FEATURES             Location/Qualifiers
     source          1..2069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgaggc gcctcaggtg gtggagatcg acccggactt cgagccgctg
       61 ccccggccgc gctcgtgcac ctggccgctg cccaggccgg agtttagcca gtccaactcg
      121 gccacctcca gcccggcgcc gtcgggcagc gcggctgcca accccgacgc cgcggcgggc
      181 ctgccctcgg cctcggctgc cgctgtcagc gccgacttca tgagcaacct gagcttgctg
      241 gaggagagcg aggacttccc gcaggcgccc ggctccgtgg cggcggcggt ggcggcggcg
      301 gccgccgcgg ccgccaccgg ggggctgtgc ggggacttcc agggcccgga ggcgggctgc
      361 ctgcacccag cgccaccgca gcccccgccg cccgggccgc tgtcgcagca cccgccggtg
      421 ccccccgccg ccgctgggcc gctcgcgggg cagccgcgca agagcagctc gtcccgccgc
      481 aacgcgtggg gcaacctgtc ctacgccgac ctcatcacca aggccatcga gagctcggcg
      541 gagaagcggc tcacgctgtc gcagatctac gagtggatgg tcaagagcgt gccctacttc
      601 aaggataagg gtgacagcaa cagctcggcg ggctggaaga attcaattcg tcataatctg
      661 tccctacaca gcaagttcat tcgtgtgcag aatgaaggaa ctggaaaaag ttcttggtgg
      721 atgctcaatc cagagggtgg caagagcggg aaatctccta ggagaagagc tgcatccatg
      781 gacaacaaca gtaaatttgc taagagccga agccgagctg ccaagaagaa agcatctctc
      841 cagtctggcc aggagggtgc tggggacagc cctggatcac agttttccaa atggcctgca
      901 agccctggct ctcacagcaa tgatgacttt gataactgga gtacatttcg ccctcgaact
      961 agctcaaatg ctagtactat tagtgggaga ctctcaccca ttatgaccga acaggatgat
     1021 cttggagaag gggatgtgca ttctatggtg tacccgccat ctgccgcaaa gatggcctct
     1081 actttaccca gtctgtctga gataagcaat cccgaaaaca tggaaaatct tttggataat
     1141 ctcaaccttc tctcatcacc aacatcatta actgtttcga cccagtcctc acctggcacc
     1201 atgatgcagc agacgccgtg ctactcgttt gcgccaccaa acaccagttt gaattcaccc
     1261 agcccaaact accaaaaata tacatatggc caatccagca tgagcccttt gccccagatg
     1321 cctatacaaa cacttcagga caataagtcg agttatggag gtatgagtca gtataactgt
     1381 gcgcctggac tcttgaagga gttgctgact tctgactctc ctccccataa tgacattatg
     1441 acaccagttg atcctggggt agcccagccc aacagccggg ttctgggcca gaacgtcatg
     1501 atgggcccta attcggtcat gtcaacctat ggcagccagg catctcataa caaaatgatg
     1561 aatcccagct cccataccca ccctggacat gctcagcaga catctgcagt taacgggcgt
     1621 cccctgcccc acacggtaag caccatgccc cacacctcgg gtatgaaccg cctgacccaa
     1681 gtgaagacac ctgtacaagt gcctctgccc caccccatgc agatgagtgc cctggggggc
     1741 tactcctccg tgagcagctg caatggctat ggcagaatgg gccttctcca ccaggagaag
     1801 ctcccaagtg acttggatgg catgttcatt gagcgcttag actgtgacat ggaatccatc
     1861 attcggaatg acctcatgga tggagataca ttggatttta actttgacaa tgtgttgccc
     1921 aaccaaagct tcccacacag tgtcaagaca acgacacata gctgggtgtc aggctagtaa
     1981 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     2041 gacgaagatt attagatgct tgcctcggt
//
