LOCUS       sequence-280100-        1619 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0591
FEATURES             Location/Qualifiers
     source          1..1619
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatccggg gaatgagaat tcagccacag aggctgccgc gatcatagac
       61 ctagatcccg acttcgaacc ccagagccgt ccccgctcct gcacctggcc ccttccccga
      121 ccagagatcg ctaaccagcc gtccgagccg cccgaggtgg agccagatct gggggaaaag
      181 gtacacacgg aggggcgctc agagccgatc ctgttgccct ctcggctccc agagccggcc
      241 gggggccccc agcccggaat cctgggggct gtaacaggtc ctcggaaggg aggctcccgc
      301 cggaatgcct ggggaaatca gtcatatgca gaactcatca gccaggccat tgaaagcgcc
      361 ccggagaagc gactgacact tgcccagatc tacgagtgga tggtccgtac tgtaccctac
      421 ttcaaggaca agggtgacag caacagctca gcaggatgga agaactcgat ccgccacaac
      481 ctgtccctgc acagcaagtt catcaaggtt cacaacgagg ccaccggcaa aagctcttgg
      541 tggatgctga accctgaggg aggcaagagc ggcaaagccc cccgccgccg ggccgcctcc
      601 atggatagca gcagcaagct gctccggggc cgcagtaaag cccccaagaa gaaaccatct
      661 gtgctgccag ctccacccga aggtgccact ccaacgagcc ctgtcggcca ctttgccaag
      721 tggtcaggca gcccttgctc tcgaaaccgt gaagaagccg atatgtggac caccttccgt
      781 ccacgaagca gttcaaatgc cagcagtgtc agcacccggc tgtccccctt gaggccagag
      841 tctgaggtgc tggcggagga aataccagct tcagtcagca gttatgcagg gggtgtccct
      901 cccaccctca atgaaggtct agagctgtta gatgggctca atctcacctc ttcccattcc
      961 ctgctatctc ggagtggtct ctctggcttc tctttgcagc atcctggggt taccggcccc
     1021 ttacacacct acagcagctc ccttttcagc ccagcagagg ggcccctgtc agcaggagaa
     1081 gggtgcttct ccagctccca ggctctggag gccctgctca cctctgatac gccaccaccc
     1141 cctgctgacg tcctcatgac ccaggtagat cccattctgt cccaggctcc gactcttctg
     1201 ttgctggggg ggcttccttc ctccagtaag ctggccacgg gcgtcggcct gtgtcccaag
     1261 cccctagagg ctccaggccc cagcagtctg gttcccaccc tttctatgat agcaccacct
     1321 ccagtcatgg caagtgcccc catccccaag gctctgggga ctcctgtgct cacaccccct
     1381 actgaagctg caagccaaga cagaatgcct caggatctag atcttgatat gtatatggag
     1441 aacctggagt gtgacatgga taacatcatc agtgacctca tggatgaggg cgagggactg
     1501 gacttcaact ttgagccaga tccctagtaa tgaactagtc cgtaacttga aagtatttcg
     1561 atttcttggc tttatatatc ttgtggaaag gacgaaggcg attattgtgg acagagggt
//
