LOCUS       sequence-280112-        1781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0606
FEATURES             Location/Qualifiers
     source          1..1781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgacggc gaccatagct ctccaggtca atggccagca aggagggggg
       61 tccgagccgg cggcggcggc ggcagtggtg gcagcgggag acaaatggaa acctccacag
      121 ggcacagact ccatcaagat ggagaacggg cagagcacag ccgccaagct ggggctgcct
      181 cccctgacgc ccgagcagca ggaggccctt cagaaggcca agaagtacgc catggagcag
      241 agcatcaaga gtgtgctggt gaagcagacc atcgcgcacc agcagcagca gctcaccaac
      301 ctgcagatgg cagcagtgac aatgggcttt ggagatcctc tctcaccttt gcaatcgatg
      361 gcggctcagc ggcagcgggc gctggccatc atgtgccgcg tctacgtggg ctctatctac
      421 tatgagctgg gggaggacac catccgccag gcctttgccc cctttggccc catcaagagc
      481 atcgacatgt cctgggactc cgtcaccatg aagcacaagg gctttgcctt cgtggagtat
      541 gaggtccccg aagctgcaca gctggccttg gagcagatga actcggtgat gctggggggc
      601 aggaacatca aggtgggcag acccagcaac atagggcagg cccagcccat catagaccag
      661 ttggctgagg aggcacgggc cttcaaccgc atctacgtgg cctctgtgca ccaggacctc
      721 tcagacgatg acatcaagag cgtgtttgag gcctttggca agatcaagtc ctgcacactg
      781 gcccgggacc ccacaactgg caagcacaag ggctacggct tcattgagta cgagaaggcc
      841 cagtcgtccc aagatgctgt gtcttccatg aacctctttg acctgggtgg ccagtacttg
      901 cgggtgggca aggctgtcac accgcccatg cccctactca caccagccac gcctggaggc
      961 ctcccacctg ccgctgctgt ggcagctgct gcagccactg ccaagatcac agctcaggaa
     1021 gcagtggccg gagcagcggt gctgggtacc ctgggcacac ctggactggt gtccccagca
     1081 ctgaccctgg cccagcccct gggcactttg ccccaggctg tcatggctgc ccaggcacct
     1141 ggagtcatca caggtgtgac cccagcccgt cctcctatcc cggtcaccat cccctcggtg
     1201 ggagtggtga accccatcct ggccagccct ccaacgctgg gtctcctgga gcccaagaag
     1261 gagaaggaag aagaggagct gtttcccgag tcagagcggc cagagatgct gagcgagcag
     1321 gagcacatga gcatctcggg cagtagcgcc cgacacatgg tgatgcagaa gctgctccgc
     1381 aagcaggagt ctacagtgat ggttctgcgc aacatggtgg accccaagga catcgatgat
     1441 gacctggaag gggaggtgac agaggagtgt ggcaagttcg gggccgtgaa ccgcgtcatc
     1501 atctaccaag agaaacaagg cgaggaggag gatgcagaaa tcattgtcaa gatctttgtg
     1561 gagttttcca tagcctctga gactcataag gccatccagg ccctcaatgg ccgctggttt
     1621 gctggccgca aggtggtggc tgaagtgtac gaccaggagc gttttgataa cagtgacctc
     1681 tctgcgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1741 tcttgtggaa aggacgaagt ccctgtactg agtgcagaca a
//
