LOCUS       sequence-280115-        1163 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0608
FEATURES             Location/Qualifiers
     source          1..1163
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             604..633
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     regulatory      630..639
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgcgga gctggcgatg ggcgccgagc tgcccagcag cccgctggcc
       61 atcgagtacg tcaacgactt cgacctgatg aagttcgagg tgaagaagga gcctcccgag
      121 gccgagcgct tctgccaccg cctgccgcca ggctcgctgt cctcgacgcc gctcagcacg
      181 ccctgctcct ccgtgccctc ctcgcccagc ttctgcgcgc ccagcccggg caccggcggc
      241 ggcggcggcg cggggggcgg cggcggctcg tctcaggccg ggggcgcccc cgggccgccg
      301 agcgggggcc ccggcgccgt cgggggcacc tcggggaagc cggcgctgga ggatctgtac
      361 tggatgagcg gctaccagca tcacctcaac cccgaggcgc tcaacctgac gcccgaggac
      421 gcggtggagg cgctcatcgg cagcggccac cacggcgcgc accacggcgc gcaccacccg
      481 gcggccgccg cagcctacga ggctttccgc ggcccgggct tcgcgggcgg cggcggagcg
      541 gacgacatgg gcgccggcca ccaccacggc gcgcaccacg ccgcccacca tcaccacgcc
      601 gcccaccacc accaccacca ccaccaccac catggcggcg cgggacacgg cggtggcgcg
      661 ggccaccacg tgcgcctgga ggagcgcttc tccgacgacc agctggtgtc catgtcggtg
      721 cgcgagctga accggcagct ccgcggcttc agcaaggagg aggtcatccg gctcaagcag
      781 aagcggcgca cgctcaagaa ccgcggctac gcgcagtcct gccgcttcaa gcgggtgcag
      841 cagcggcaca ttctggagag cgagaagtgc caactccaga gccaggtgga gcagctgaag
      901 ctggaggtgg ggcgcctggc caaagagcgg gacctgtaca aggagaaata cgagaagctg
      961 gcgggccggg gcggccccgg gagcgcgggc ggggccggtt tcccgcggga gccttcgccg
     1021 ccgcaggccg gtcccggcgg ggccaagggc acggccgact tcttcctgta gtaatgaact
     1081 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgac
     1141 agtaatctga ggcgccgcga gcc
//
