LOCUS       sequence-280118-         509 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0609
FEATURES             Location/Qualifiers
     source          1..509
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggggctgtc ggtgcgcgag ctgaaccggc atctgcgcgg gctctccgcc
       61 gaggaggtga cacggctcaa gcagcggcgc cgcacactca aaaaccgtgg ctacgccgcc
      121 agctgccgcg tgaagcgcgt gtgccagaag gaggagctgc agaagcagaa gtcggagctg
      181 gagcgcgagg tggacaagct ggcgcgcgag aacgccgcca tgcgcctgga gctcgacgcg
      241 ctgcgcggca agtgcgaggc gctgcagggc ttcgcgcgct ccgtggccgc cgcccgcggg
      301 cccgccacgc tcgtggcgcc ggccagcgtc atcaccatcg tcaagtccac cccgggctcg
      361 gggtctggcc ccgcccacgg cccggacccc gcccacggcc cggcctcctg ctcctagtaa
      421 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
      481 gacgagaaag aacctcgagt tgcggccgt
//
