LOCUS       sequence-280124-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0615
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggctca taatgagggc tctaacttgg aaaggcatca ggccaagccc
       61 aaagagaaga ttgagtataa atgctcagaa cgtgagcaga gattcatcca gcacttggac
      121 ctgattgaac atgcgagtac acacacggga aagaaactct gcgagtctga tgtgtgtcag
      181 agttccagtc ttacaggaca taagaaagtc ctctctagag agaaaggtca tcagtgtcat
      241 gagtgtggga aagcctttca gaggagttca cacctcgtca gacatcagaa aatccatctt
      301 ggtgagaagc cttatcagtg caatgagtgt ggcaaagtct ttagccagaa tgcaggcctt
      361 ttggaacatc tcagaattca tactggagag aaaccttatc tatgtatcca ttgtggaaaa
      421 aattttaggc gcagctctca ccttaatcga catcagagaa ttcacagtca ggaggagccc
      481 tgtgagtgca aggagtgtgg aaaaaccttt agtcaggcct tactcctcac ccaccatcag
      541 agaatccata gtcactccaa aagccatcaa tgtaacgagt gtggaaaagc tttcagtttg
      601 acctcagacc ttattcgaca ccacagaatt catactggag aaaaaccttt caagtgtaac
      661 atatgccaga aagccttccg actaaactca caccttgctc agcatgtaag aatccacaat
      721 gaagaaaaac cctatcagtg tagtgaatgt ggagaagcct tcaggcaaag gtcaggtctt
      781 tttcaacatc agagatatca ccacaaagac aaactggctt agtaatgaac tagtccgtaa
      841 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga actctcggaa
      901 taagggccat ggag
//
