LOCUS       sequence-280127-        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0616
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcaacagc attggaccca gaccagccaa agaaacaaaa aatacttgtg
       61 gaggagatgg cccctctgaa aggagtacag gaacagcagg ttcggcatga gtgtgaagtt
      121 acaaagcctg agaaagagaa gggtgaggag acaaggattg agaatgggaa gcttattgta
      181 gtaacagact cttgtggaag agtagagtca tctgggaaaa tatctgaacc catggaggct
      241 cataatgagg gctctaactt ggaaaggcat caggccaagc ccaaagagaa gattgagtat
      301 aaatgctcag aacgtgagca gagattcatc cagcacttgg acctgattga acatgcgagt
      361 acacacacgg gaaagaaact ctgcgagtct gatgtgtgtc agagttccag tcttacagga
      421 cataagaaag tcctctctag agagaaaggt catcagtgtc atgagtgtgg gaaagccttt
      481 cagaggagtt cacacctcgt cagacatcag aaaatccatc ttggtgagaa gccttatcag
      541 tgcaatgagt gtggcaaagt ctttagccag aatgcaggcc ttttggaaca tctcagaatt
      601 catactggag agaaacctta tctatgtatc cattgtggaa aaaattttag gcgcagctct
      661 caccttaatc gacatcagag aattcacagt caggaggagc cctgtgagtg caaggagtgt
      721 ggaaaaacct ttagtcaggc cttactcctc acccaccatc agagaatcca tagtcactcc
      781 aaaagccatc aatgtaacga gtgtggaaaa gctttcagtt tgacctcaga ccttattcga
      841 caccacagaa ttcatactgg agaaaaacct ttcaagtgta acatatgcca gaaagccttc
      901 cgactaaact cacaccttgc tcagcatgta agaatccaca atgaagaaaa accctatcag
      961 tgtagtgaat gtggagaagc cttcaggcaa aggtcaggtc tttttcaaca tcagagatat
     1021 caccacaaag acaaactggc ttagtaatga actagtccgt aacttgaaag tatttcgatt
     1081 tcttggcttt atatatcttg tggaaaggac gacacgccaa gagtatacct gcagga
//
