LOCUS       sequence-28013-e         495 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Hakre S, Tussie-Luna MI, Ashworth T, Novina CD, Settleman J, Sharp 
            PA, Roy AL
  TITLE     Opposing functions of TFII-I spliced isoforms in growth 
            factor-induced gene expression.
  JOURNAL   Mol Cell. 2006 Oct 20. 24(2):301-8.
  PUBMED    17052463
REFERENCE   2  (bases 1 to 495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2006 Oct 20. 24(2):301-8."
COMMENT     Sequence Label: pSFG-GFP
FEATURES             Location/Qualifiers
     source          1..495
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(24..48)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(45..83)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(116..134,135..157,158..176,177..199,200..218,219..241,
                     242..260,261..283,284..302,303..325,326..344,345..367,
                     368..386)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     complement(445..461)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
ORIGIN
        1 cagcgtggat ggcgtctcca ggcgatctga cggttcacta aacgagctct gcttatatag
       61 gcctcccacc gtacacgcct acctcgaccc gggtaccgag ctcgactttc acttttctct
      121 atcactgata gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg
      181 taaactcgac tttcactttt ctctatcact gatagggact ggtaaactcg actttcactt
      241 ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg
      301 gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta aactcgactt
      361 tcacttttct ctatcactga tagggagtgg taaactcgag ggctagagga tggtccaccc
      421 ccggggtcgg cagccttcac gtgggcggcg tgtatccaag ctgcgatgcc gtctactttg
      481 agggcggtgg gggtg
//
