LOCUS       sequence-280133-        1538 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0618
FEATURES             Location/Qualifiers
     source          1..1538
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcaacagc attggtgagt gcccattccc tggctcccct gaatctgaag
       61 aaggaggggc ttcgggtagt gagggaggat cactactcta cttgggaaca gggattcaag
      121 ctgcaaggaa acagtaaagg ccttggacag gagccattgt gcaaacaatt caggcagttg
      181 cgttatgaag agaccacagg acctcgagaa gcactaagtc ggctccggga gctctgtcaa
      241 cagtggctac agcccgagac ccataccaag gagcagatcc tggagctgct ggtgctggag
      301 cagtttctga tcatcctgcc taaggagctc caggcccggg tgcaggagca tcacccagag
      361 agcagggagg acgtggttgt tgttctggag gatttgcagc tggatcttgg agaaacagga
      421 caacaggacc cagaccagcc aaagaaacaa aaaatacttg tggaggagat ggcccctctg
      481 aaaggagtac aggaacagca ggttcggcat gagtgtgaag ttacaaagcc tgagaaagag
      541 aagggtgagg agacaaggat tgagaatggg aagcttattg tagtaacaga ctcttgtgga
      601 agagtagagt catctgggaa aatatctgaa cccatggagg ctcataatga gggctctaac
      661 ttggaaaggc atcaggccaa gcccaaagag aagattgagt ataaatgctc agaacgtgag
      721 cagagattca tccagcactt ggacctgatt gaacatgcga gtacacacac gggaaagaaa
      781 ctctgcgagt ctgatgtgtg tcagagttcc agtcttacag gacataagaa agtcctctct
      841 agagagaaag gtcatcagtg tcatgagtgt gggaaagcct ttcagaggag ttcacacctc
      901 gtcagacatc agaaaatcca tcttggtgag aagccttatc agtgcaatga gtgtggcaaa
      961 gtctttagcc agaatgcagg ccttttggaa catctcagaa ttcatactgg agagaaacct
     1021 tatctatgta tccattgtgg aaaaaatttt aggcgcagct ctcaccttaa tcgacatcag
     1081 agaattcaca gtcaggagga gccctgtgag tgcaaggagt gtggaaaaac ctttagtcag
     1141 gccttactcc tcacccacca tcagagaatc catagtcact ccaaaagcca tcaatgtaac
     1201 gagtgtggaa aagctttcag tttgacctca gaccttattc gacaccacag aattcatact
     1261 ggagaaaaac ctttcaagtg taacatatgc cagaaagcct tccgactaaa ctcacacctt
     1321 gctcagcatg taagaatcca caatgaagaa aaaccctatc agtgtagtga atgtggagaa
     1381 gccttcaggc aaaggtcagg tctttttcaa catcagagat atcaccacaa agacaaactg
     1441 gcttagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1501 tgtggaaagg acgaagattg cgcctcagtt cgacgcca
//
