LOCUS       sequence-280136-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0623
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagcttgt tccagccact ccccattatc ctgcagatgt gcttttccag
       61 actgatccaa ctgcagagat ggcagctgag tcattgcctt tctccttcgg gacactgtcc
      121 agctgggagc tggaagcctg gtatgaggac ctgcaagagg tcctgtcttc agatgaaaat
      181 gggggtacct atgtttcacc tcctggaaat gaagaggaag aatcaaaaat cttcaccact
      241 cttgaccctg cttctctggc ttggctgact gaggaggagc cagaaccagc agaggtcaca
      301 agcacctccc agagccctca ctctccagat tccagtcaga gctccctggc tcaggaggaa
      361 gaggaggaag accaagggag aaccaggaaa cggaaacaga gtggtcattc cccagcccgg
      421 gctggaaagc agcgcatgaa ggagaaagaa caggagaatg aaaggaaagt ggcacagcta
      481 gctgaagaga atgaacggct caagcaggaa atcgagcgcc tgaccaggga agtagaggcg
      541 actcgccgag ctctgattga ccgaatggtg aatctgcacc aagcatagta atgaactagt
      601 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgagcga
      661 ggaaagttac cagtcttgcg
//
