LOCUS       sequence-280148-        6002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0657
FEATURES             Location/Qualifiers
     source          1..6002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgccacca tgaaggcggc agacactgtg atcctgtggg caagaagtaa aaatgaccag
       61 ctgaggattt cttttcctcc aggactgtgt tggggtgaca ggatgcctga taaggatgac
      121 attcggctgc tgccgtcagc attgggtgtg aagaagagaa aacgaggacc caagaagcag
      181 aaggagaaca agccaggaaa accccgaaaa cgcaagaagc gtgacagtga ggaggaattt
      241 ggttctgagc gagatgagta ccgggagaag tcagagagtg ggggcagtga atatggaacc
      301 ggaccgggtc ggaaacgaag aaggaagcac cgagaaaaaa aggagaagaa gacaaagcgg
      361 cggaaaaagg gggagggaga tggggggcaa aagcaagtgg aacagaagtc atcagcaact
      421 ctgcttctga cctggggcct ggaggatgtg gagcatgtgt tctctgagga ggattaccac
      481 acgctcacca actacaaagc cttcagccag ttcatgaggc ccctaattgc taagaagaat
      541 cctaagatcc caatgtctaa gatgatgacc atccttgggg ccaaatggag agagttcagt
      601 gccaacaacc ccttcaaggg gtcagcagct gctgtggcgg cggcagcggc agcagcagca
      661 gcagctgtag ctgagcaggt gtcagctgct gtctcgtcgg ccacccccat agcaccctcc
      721 ggaccccccg cccttccacc accccctgct gctgatatcc agcccccacc catccgaaga
      781 gccaaaacca aagagggcaa aggtccaggc cataagaggc ggagtaagag cccccgagtg
      841 cctgatggac gcaagaagct tcggggaaag aaaatggcac cactcaaaat aaaactaggg
      901 cttctgggtg gcaagaggaa gaaaggaggc tcgtatgttt ttcagagcga cgaaggtcct
      961 gaaccagagg ctgaggaatc agacctggac agtggcagtg tccacagtgc ctcaggccgg
     1021 cctgatggcc ctgtccgcac caagaaacta aagagaggcc ggccaggaag gaagaagaag
     1081 aaggtcctgg gctgtcctgc agtggccggg gaggaggagg ttgatggcta cgagacggat
     1141 caccaggatt actgtgaggt gtgccagcag ggtggggaaa ttattctgtg tgacacctgc
     1201 cctcgtgcct accacctcgt ctgccttgat cctgagcttg accgggctcc agagggcaaa
     1261 tggagctgcc ctcactgtga gaaggagggg gtccagtggg aggccaagga ggaagaagaa
     1321 gaatacgaag aggagggaga ggaagaaggg gagaaggagg aggaggatga tcacatggag
     1381 tactgccgcg tatgcaagga cggcggggag ctcctgtgct gtgacgcgtg catctcctcc
     1441 taccacattc attgtctaaa ccctcccctg cctgacattc ccaatggtga atggctgtgt
     1501 ccccgatgca catgccccgt gctgaagggt cgagtgcaga agatcctaca ttggcggtgg
     1561 ggggagccac ctgtagcagt gccagcccct caacaggcag atggaaatcc agatgtccca
     1621 cccccccgtc ctcttcaagg cagatcagag cgagagttct ttgtcaagtg ggtaggacta
     1681 tcctactggc actgctcctg ggccaaggag cttcagctgg aaatcttcca tttggttatg
     1741 tatcgaaact accagcggaa gaatgacatg gatgagcccc cacccctgga ctatggctcc
     1801 ggcgaggatg atgggaagag cgacaagcgt aaagtgaaag acccgcacta tgctgagatg
     1861 gaggagaagt actatcgttt tggcatcaag ccagagtgga tgaccgtcca ccgcatcatc
     1921 aaccacagtg tggataaaaa ggggaattac cactatctag taaaatggag ggacttacca
     1981 tatgaccagt ccacgtggga ggaagatgaa atgaatatcc ctgaatacga agaacataag
     2041 caaagctact ggagacaccg agaactaatt atgggggaag accctgccca gccccgcaag
     2101 tataagaaga agaagaagga gctacagggt gatgggcctc ccagttctcc cactaatgat
     2161 cctaccgtga aatatgagac tcagccacgg tttatcacag ccactggagg caccctgcac
     2221 atgtatcagt tggaagggct gaactggcta cgcttctcct gggcccaggg cactgacacc
     2281 attctagctg atgagatggg gctaggcaag accatacaaa ccatcgtctt cctctactca
     2341 ctctacaagg agggccacac aaaaggtccc ttcctggtga gtgccccact ctctaccatc
     2401 attaactggg agcgggagtt ccagatgtgg gcacccaaat tctatgtggt gacatacacg
     2461 ggtgacaagg acagccgggc catcattcgt gagaatgaat tctcctttga ggacaatgcc
     2521 atcaaagggg gcaagaaagc ttttaagatg aagagggagg cacaggtgaa gttccatgtt
     2581 ctcctgacat cgtatgagct gatcaccatt gatcaggcag cacttggttc catccgctgg
     2641 gcctgtcttg tggtagatga ggcccatcga ctcaagaaca accagtccaa gtttttcagg
     2701 gttctcaatg gttacaagat agatcataag ttgctgctga caggaacccc attgcagaat
     2761 aatctggagg agctcttcca tctcctgaac ttcctcaccc cagagagatt taacaacttg
     2821 gagggcttcc tggaggagtt tgctgacata tccaaagagg accagatcaa gaaactgcat
     2881 gatttgctgg ggccacacat gctgcggaga ctcaaggcag atgtctttaa gaacatgcca
     2941 gccaagacag agctcatcgt tcgggtggag ctaagcccca tgcagaagaa atactacaaa
     3001 tacatcctga ctcgaaattt tgaggccttg aattcacgag gtggtgggaa ccaggtgtcg
     3061 ctgcttaata tcatgatgga tcttaagaag tgctgcaacc atccatacct ttttcccgtg
     3121 gctgctatgg agtcccccaa actccccagt ggggcttatg agggtggggc acttattaag
     3181 tcgtctggga agctcatgct gctccagaag atgctgcgaa agctgaagga gcaaggacac
     3241 cgagtgctca tcttctcgca gatgaccaaa atgttagact tgcttgagga cttcttagac
     3301 tatgaaggct acaagtatga gcgcatcgat ggtggtatca cgggtgccct gaggcaggag
     3361 gccatcgatc ggtttaatgc tcctggggcc caacaattct gcttcctcct gtccacccga
     3421 gctgggggcc tgggcatcaa tctggccact gctgacactg tcatcatctt tgattctgac
     3481 tggaaccccc ataatgacat ccaggccttt agccgggctc atcggattgg ccaggccaac
     3541 aaagtgatga tttaccggtt tgtgactcgc gcgtcagtgg aagagcgaat cacacaagtg
     3601 gccaagagaa agatgatgct gacacacctg gttgtgcggc ctgggctggg ctccaaggca
     3661 ggctccatgt ccaagcagga gcttgacgac attctcaaat ttggcactga agagctattc
     3721 aaggatgaaa acgaggggga gaacaaggag gaggacagca gtgtgattca ttatgacaat
     3781 gaggccatcg ctcggctgtt ggaccggaac caggatgcaa ctgaggacac tgacgtgcag
     3841 aacatgaatg agtatctcag ctccttcaag gtggcacagt acgtcgtgcg ggaagaagac
     3901 aagattgagg aaattgagcg agagatcatc aagcaggagg agaatgtgga ccctgactac
     3961 tgggagaagc tgctgaggca tcactatgag caacagcagg aagacctagc ccggaatcta
     4021 ggcaagggca agcgggttcg caagcaagtt aactacaatg atgctgctca ggaagaccaa
     4081 gacaaccagt cagagtactc ggtgggttca gaggaggagg atgaagactt cgatgaacgt
     4141 cctgaagggc gtagacagtc aaagaggcag ctccggaatg agaaagataa gccactgcct
     4201 ccactgctgg cccgagtcgg gggcaacatt gaggtgctgg gcttcaacac ccgtcagcgg
     4261 aaggctttcc tcaatgctgt gatgcgctgg gggatgccac cacaggatgc cttcaccaca
     4321 cagtggctgg tgcgggacct gaggggcaag actgagaagg agtttaaggc ctatgtgtct
     4381 ttgttcatgc gccatctgtg tgagcctggg gcagacggct ctgaaacctt tgccgatggg
     4441 gtccctcggg agggactgag tcgccagcag gtgttgaccc gcattggagt catgtctctc
     4501 gtcaaaaaga aggtgcagga gtttgagcac atcaatgggc gttggtcaat gccggaactg
     4561 atgcctgacc ccagcgccga ttctaagcgc tcctccagag cctcctctcc taccaaaacg
     4621 tctcccacca ctcctgaggc ttctgctacc aacagtccct gcacctctaa acctgctact
     4681 ccagctccaa gtgagaaagg agaaggcata aggacacctc ttgagaagga ggaagctgaa
     4741 aaccaggagg aaaagccaga gaagaacagc agaattgggg agaagatgga gacagaggct
     4801 gatgccccca gcccagcccc atcacttggg gagcggctgg agccaaggaa gattcctcta
     4861 gaggatgagg tgccaggggt gcctggagag atggagcctg aacctgggta ccgtggggac
     4921 agagagaagt cagaagatgt aaaaggtgac cgggagcttc gaccagggcc tcgagatgag
     4981 ccacggtcca atgggcgacg agaggaaaag acagagaagc cccggttcat gttcaatatc
     5041 gccgatggtg gcttcacaga gcttcacaca ctgtggcaga atgaggaacg ggcagctatt
     5101 tcctcgggga aactcaatga gatctggcac agaagacatg actattggct tctggctggg
     5161 attgtcctcc atggctatgc acggtggcag gacatccaga atgatgctca atttgccatt
     5221 atcaacgagc catttaaaac tgaagccaat aaggggaact ttctggagat gaaaaataag
     5281 ttcctggccc ggaggttcaa gctcctggag caggcgctgg tgattgagga gcagctgcgg
     5341 cgggcggcct acctgaacct gtcgcaggag ccggcgcacc ccgccatggc cctccacgcc
     5401 cgcttcgccg aggccgagtg cctggccgag agccaccagc acctctccaa ggagtcgctg
     5461 gcggggaaca agccggccaa cgccgtcctg cacaaggttc tgaaccagct ggaggagttg
     5521 ctgagcgaca tgaaggcgga cgtgacccgc ctgccagcca cgctgtcccg aatacccccc
     5581 atcgcagccc gccttcagat gtccgagcgc agcatcctca gccggctggc cagcaagggc
     5641 acggagcctc accccacacc ggcctacccg ccgggtccct acgctacacc tccggggtac
     5701 ggggcggcct tcagcgccgc acccgtaggg gccctggccg ccgcaggcgc caattacagc
     5761 cagatgcctg cagggtcctt catcacagcc gccaccaacg gccctccagt gcttgtgaag
     5821 aaggagaagg aaatggtggg ggcattggtg tcagacgggc tggatcggaa ggagccccga
     5881 gccggggagg tgatctgtat agacgactag taatgaacta gtccgtaact tgaaagtatt
     5941 tcgatttctt ggctttatat atcttgtgga aaggacgaga caatagagac agtttgacag
     6001 ac
//
