LOCUS       sequence-280151-        2948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0660
FEATURES             Location/Qualifiers
     source          1..2948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      351..360
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagatcc aggaatgatg agtctttttg gcgaggatgg gaatattttc
       61 agtgaaggtc ttgaaggcct cggagaatgt ggttacccgg aaaatccagt aaatcctatg
      121 ggtcagcaaa tgccaataga ccaaggcttt gcctctttac agccatccct tcatcatcct
      181 tcaactaatc aaaatcaaac aaagctgaca cattttgatc actataatca gtatgaacaa
      241 caaaagatgc atctgatgga tcagccgaac agaatgatga gcaacacccc tgggaacgga
      301 ctcgcgtctc cgcactcgca gtatcacacc cctcccgttc ctcaggtgcc ccatggtggc
      361 agtggtggcg gtcagatggg tgtctaccct ggcatgcaga atgagaggca tgggcaatcc
      421 tttgtggaca gcagctccat gtggggcccc agggctgttc aggtaccaga ccagatacga
      481 gccccctacc agcagcagca gccacagccg cagccaccgc agccggctcc gtcggggccc
      541 cctgcacagg gccaccctca gcacatgcag cagatgggca gctatatggc acgtggggat
      601 ttttccatgc agcagcatgg tcagccacag cagaggatga gccagttttc ccaaggccaa
      661 gagggcctca atcagggaaa tccttttatt gccacctcag gacctggcca cttgtcccac
      721 gtgccccagc agagtcccag catggcacct tccttgcgtc actcggtgca gcagttccat
      781 caccacccct ctactgctct ccatggagaa tccgttgccc acagtcccag attctccccg
      841 aatcctcccc aacaaggggc tgttaggccg caaaccctta actttagttc tcggagccag
      901 acagtcccct ctcctactat aaacaactca gggcagtatt ctcgatatcc ttacagtaac
      961 ctaaatcagg gattagttaa caatacaggg atgaatcaaa atttaggcct tacaaataat
     1021 actccaatga atcagtccgt accaagatac cccaatgctg taggattccc atcaaacagt
     1081 ggtcaaggac taatgcacca gcagcccatc caccccagtg gctcacttaa ccaaatgaac
     1141 acacaaacta tgcatccttc acagcctcag ggaacttatg cctctccacc tcccatgtca
     1201 cccatgaaag caatgagtaa tccagcaggc actcctcctc cacaagtcag gccgggaagt
     1261 gctgggatac caatggaagt tggcagttat ccaaatatgc cccatcctca gccatctcac
     1321 cagccccctg gtgccatggg aatcggacag aggaatatgg gccccagaaa catgcagcag
     1381 tctcgtccat ttataggcat gtcctcggca ccaagggaat tgactgggca catgaggcca
     1441 aatggttgtc ctggtgttgg ccttggagac ccacaagcaa tccaggaacg actgatacct
     1501 ggccaacaac atcctggtca acagccatct tttcagcagt tgccaacctg tcctccactg
     1561 cagcctcacc cgggcttgca ccaccagtct tcacctccac accctcatca ccagccttgg
     1621 gcacagctcc acccatcacc ccagaacacc ccgcagaaag tgcctgtgca tcagcattcc
     1681 ccgtcggagc cctttctaga gaaaccagtg ccggatatga ctcagaaacc gaaacagaaa
     1741 cgacatagat gtcgaaaccc taataaattg gatataaaca ctttgacagg agaagaaagg
     1801 gtgcctgttg tcaataaacg aaatgggaag aagatgggtg gagctatggc gcctccaatg
     1861 aaggatctac ccaggtggct ggaagaaaat cctgaatttg cagttgctcc agactggact
     1921 gatatagtta agcagtctgg ttttgttcct gagtcgatgt ttgaccgcct tctcactggg
     1981 cctgtagtgc ggggagaggg agcgagcaga agaggaagaa ggcccaaaag tgagatcgcc
     2041 agagcagccg cggccgccgc tgctgtggcc tccacgtcag ggatcaaccc tttgctggtg
     2101 aacagcctgt ttgctggaat ggacctgacg agccttcaga atctccagaa tctccagtcg
     2161 ctccagctgg caggcctcat gggcttccct ccaggactgg caacagctgc caccgccgga
     2221 ggcgatgcga agaaccctgc tgctgtgctg cccctgatgc tgccaggaat ggcgggcctg
     2281 cccaacgtgt ttggcttggg cgggctgttg aataaccctc tgtcagctgc tactggaaac
     2341 accactactg cttctagtca aggagaaccg gaagacagca cttcaaaagg agaggagaaa
     2401 ggaaatgaga atgaagacga gaacaaagac tctgagaaaa gcacagatgc tgtttcggct
     2461 gctgactctg cgaatggatc tgttggtgct gctactgccc cggctggatt gccctcaaac
     2521 ccgctagcct tcaacccttt cctcctgtcc acaatggccc cgggcctctt ctacccatcc
     2581 atgtttctac ctccaggact ggggggattg acgctgcctg ggttcccagc attggcagga
     2641 cttcagaatg ccgtgggctc cagcgaagaa aaggctgctg acaaggctga gggaggaccc
     2701 tttaaagatg gagagaccct tgaaggcagc gatgccgagg agagcctgga taagactgca
     2761 gagtcctccc tcttagaaga cgaaatagca cagggtgaag agctagactc acttgatggg
     2821 ggggatgaaa tagaaaacaa tgaaaatgat gaatagtaat gaactagtcc gtaacttgaa
     2881 agtatttcga tttcttggct ttatatatct tgtggaaagg acgacagata ggtaagtgtc
     2941 actgatta
//
