LOCUS       sequence-280172-        1220 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0711
FEATURES             Location/Qualifiers
     source          1..1220
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggattcagt ggtctttgag gatgtagatg tgaacttcac ccaggaggag
       61 tgggctttgc tggatccttc ccagaagaat ctctacagag atgtgatgca ggaaaccttc
      121 aggaacctgg cttctgtagg aaaaaaatgg aaagatcaga aaattgaaga tgagtacaaa
      181 aatcccagga gaaacctaag aggtcttatg ggagagagac tcttagaaag taagaaagat
      241 catcagcatg gagaaatttt gacccaggtt ccagatgaca tgctgaagaa gaaaactccc
      301 cgagtaaaat catgtggaga agtcagcgtg ggtcatgcat cccttaatag gcaccacaga
      361 gctgacactg gacacaagcc atatgagtat caggaatatg gacagaagcc atataaatgt
      421 acatactgta agaaagcctt cagtgatctc ccctactttc gaacacatga atgggctcac
      481 actgggggga aaccttatga ttgtgaggaa tgtggaaaaa gctttatttc ccgttcaagc
      541 attcgaagac acaggataat gcacagtgga gatggaccct acaaatgtaa cttttgtggg
      601 aaagccttga tgtgtctcag tttgtatctt atccacaaac gaactcacac tggagagaaa
      661 ccatatgaat gtaaacagtg tggtaaagcc tttagtcatt ctggtagcct tcgaatacat
      721 gaaagaactc acactggaga gaagccttat gaatgcagtg agtgtgggaa agcattccat
      781 agttccacat gcctccatgc acataaaata actcacactg gggagaagcc gtatgaatgt
      841 aaacagtgtg ggaaagcctt tgtttctttc aattccgttc gatatcatga aagaactcac
      901 actggagaga agccctatga atgtaagcaa tgtgggaaag ccttcagatc tgcctcgcac
      961 cttcgaacac atggaaggac tcacactgga gagaaaccct atgaatgtaa acaatgtggt
     1021 aaagcctttg gatgtgcctc gagcgttaaa atccatgaaa ggactcacac tggagaaaaa
     1081 ccctgtagct ccaacacttc gaaaggccaa ggcgagaaga ttgcttagta atgaactagt
     1141 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaacgt
     1201 gagcttcatt tcggtagtcg
//
