LOCUS       sequence-280178-        1592 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0719
FEATURES             Location/Qualifiers
     source          1..1592
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagctccc ctctcccgag tctgaggaag tccacgagcc cagattaggg
       61 gagctcttgg gaaatccaga aggtcagagc ctggggagtt ccccctctca ggacaggggc
      121 tgcaagcagg tgacagtgac ccattggaag atccagacag gagagacagc tcaagtgtgc
      181 accaagtcag gaagaaacca tattctgaac tcagaccttc ttctgcttca gagagagctc
      241 atagaggggg aagccaatcc ttgcgatatc tgtggcaaaa ccttcacgtt taattcggac
      301 ctagttaggc atcggatttc gcatgctggg gagaaacctt acacgtgcga tcagtgtggg
      361 aaaggctttg gccagagctc acaccttatg gagcatcaga gaattcacac tggagagaga
      421 ctctacgtct gtaatgtgtg tgggaaagac ttcattcact attcaggtct cattgagcat
      481 cagcgcgttc attcaggaga aaagcccttc aaatgtgcgc agtgtgggaa ggcgttttgt
      541 cacagttcag acctgattag gcaccagaga gttcacacca gagagagacc ttttgaatgc
      601 aaagagtgtg ggaaaggctt cagtcagagc tccttactta ttcgccatca gaggattcac
      661 acgggagaaa ggccctatga gtgcaatgaa tgtgggaaat ccttcataag gagctcgagc
      721 ctcattcgcc attatcagat ccacacagaa gtgaaacagt atgaatgcaa agaatgtggg
      781 aaggcattcc gtcatcgctc agaccttatt gaacaccaga gaattcacac cggagagaga
      841 ccctttgaat gcaatgagtg tgggaaagcc tttattcgga gttcaaagct cattcagcat
      901 cagaggatcc atactgggga gaggccttac gtatgcaatg agtgtgggaa gcgcttcagc
      961 cagacgtcaa acttcaccca gcatcagaga attcacactg gagagaaact ctatgaatgt
     1021 aacgagtgtg ggaaagcttt ctttctgagt tcatacctta ttcgacacca gaaaatccac
     1081 actggagaga gagtgtatga atgtaaggaa tgtgggaaag cgtttctcca gaaagcccat
     1141 ctcactgagc accagaagat ccactctggg gacaggccct tcgaatgtaa agactgtggg
     1201 aaagccttca tccagagctc caagctgctt ctgcaccaga ttattcacac tggagaaaag
     1261 ccctatgtgt gcagttattg tgggaaaggc tttattcaga ggtcaaactt ccttcaacac
     1321 cagaaaattc atactgaaga gaagctctat gaatgtagtc agtatgggag agattttaac
     1381 tcaactacaa acgttaaaaa taatcaaagg gttcaccaag agggactctc cttgagtaag
     1441 gcccccatac atttgggtga gaggtctgta gataaggggg aacacacagg taacttatag
     1501 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1561 aaggacgact gtccctatgt tgtgaaggaa ta
//
