LOCUS       sequence-28018-e         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Erdmann KS, Mao Y, McCrea HJ, Zoncu R, Lee S, Paradise S, Modregger 
            J, Biemesderfer D, Toomre D, De Camilli P
  TITLE     A role of the Lowe syndrome protein OCRL in early steps of the 
            endocytic pathway.
  JOURNAL   Dev Cell. 2007 Sep . 13(3):377-90.
  PUBMED    17765681
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2007 Sep . 13(3):377-90."
COMMENT     Sequence Label: pcDNA3-HA-human OCRL
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             97..123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtacc atggcgtacc catacgacgt cccagactac
      121 gcgggatccg agccgccgct cccggtcgga gcccagccgc ttgccactgt cgagggtatg
      181 gagatgaagg gtcctctccg ggagccctgc gccctgaccc tagcccagag gaacgggcaa
      241 tatgagttaa taatccagtt gcatgagaag gaacagcatg ttcaagatat cattcctata
      301 aatagccact tcagatgtgt tcaagaagca gaagaaactc ttttgattga catagcttct
      361 aacagtggct gcaaaattcg ggttcagggg gactggatca gagagcgccg ctttgaaatc
      421 cctgatgagg aacactgttt gaagttcctc tcagctgtcc ttgctgctca gaaagctcag
      481 tcacagcttc ttgttccaga gcaaaaggac tcatctagct ggtaccagaa attagacact
      541 aaggacaaac cttctgtttt ttcagggctt cttggatttg aagacaattt ttcttctatg
      601 aatttggaca agaaaataaa ttcacaaaat cagcctactg ggattcatcg ggaaccccca
      661 cctccaccct tttcagtgaa taaaatgctt ccacgtgaaa aagaagcttc taacaaggag
      721 cagcccaaag tgaccaacac catgcggaag ctctttgtac caaataccca atctgggcag
      781 cgggagggtc tcatcaaaca tatcctggca aagcgagaga aagaatatgt caacattcag
      841 actttcagat tttttgttgg aacttggaat gtgaatggcc agtctccaga tagcgggtta
      901 gaaccttggc tgaactgtga tcccaatcct cctgatatct actgcattgg attccaagaa
      961 ctgga
//
