LOCUS       sequence-280193-        1988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0765
FEATURES             Location/Qualifiers
     source          1..1988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggactcagt ggtctttgag gatgtggctg tggacttcac cctggaggag
       61 tgggctttgc tggattctgc tcagagggac ctctacagag atgtgatgct ggagaccttt
      121 cagaacctgg cctcagtaga tgatgaaact caatttaagg ccagtgggtc agtttctcag
      181 caggatattt atggagagaa aatacccaag gaatctaaaa tagccacgtt caccagaaat
      241 gtttcctggg cctctgtttt aggaaaaatt tgggacagtc ttagcatcga agatcaaacc
      301 acaaaccagg ggagaaatct cagtagaaat catgggttgg agagactctg tgaaagtaat
      361 gatcaatgtg gagaagccct cagccagatt ccacatctta atctgtacaa gaaaattcca
      421 cctggagtaa aacagtatga atacaacacg tacggaaaag tcttcatgca tcgccgcaca
      481 tccctcaaga gtcccatcac agttcacact ggacacaaac catatcagtg ccaggaatgt
      541 gggcaggcct acagttgtcg ttcacaccta agaatgcatg tgagaaccca caatggagag
      601 agaccctatg tgtgtaaatt atgtgggaaa acctttcctc gtacttcctc cctcaatcgg
      661 catgtaagga ttcacactgc tgagaaaacc tacgaatgta agcaatgtgg gaaagccttt
      721 attgacttct caagtcttac tagtcatctc agaagtcaca ccggagagaa gccatataag
      781 tgtaaggaat gtgggaaagc tttcagttat tcctcaacgt ttcgaagaca cacaataaca
      841 cacactggcg agaagccata taaatgtaag gaatgtgcgg aagcctttag ttattcctca
      901 acttttcgaa gacatatgat ttcacacact ggagagaagc cacataaatg taaagaatgt
      961 ggggaggcct tcagttattc ttcggctttt cgaagacaca tgataacaca cactggagag
     1021 aaaccctacg aatgcaaaca atgtgggaaa accttcattt atctccagtc ctttcgaaga
     1081 catgaaagga ttcacactgg agagaaaccc tacgaatgca aacagtgtgg gaagaccttc
     1141 atttatcccc agtcctttcg aagacatgaa aggactcatg gtggagagaa accctatgaa
     1201 tgcaaccagt gcgggaaagc attcagtcac ccctcctcct ttcgaggaca catgagggtg
     1261 cacactggag agaaacccta tgagtgcaag caatgtggga aaactttcaa ttggcccata
     1321 tctttacgaa aacatatgag aacacatact agagagaaac cctatgaatg taagcagtgt
     1381 gggaaagcct tcagcttgtc tgcttgcttt cgagaacatg tgagaatgca ccctgaagac
     1441 aaatcctatg aatgcaagct atgtgggaaa gctttctatt gccacatatc cttacaaaaa
     1501 catatgagaa gacataccgc agagaaactc tataaatgca agcagtgtgg gaaagctttc
     1561 agttggcctg aacttttgca acaacatgtg agaacgcaca ctgtagagaa gccctatgaa
     1621 tgtaaggaat gtgggaaggt cttcaaatgg ccatcatctt taccaataca tatgagactg
     1681 cacactggag agaaacctta tcaatgtaag cattgtggga aagcattcaa ttgttcctca
     1741 tccttaaggc gacatgtgag aatacacact acagaaaaac agtataagtg taatgtagga
     1801 catcctcctg caaatgaatt catgtgcagt gcttcagaaa agtcacacca ggagagagat
     1861 ctgatcaaag ttgtaaatat ggtgttgcct ttatagtaat gaactagtcc gtaacttgaa
     1921 agtatttcga tttcttggct ttatatatct tgtggaaagg acgattagct gtgttgttcc
     1981 gtttgccg
//
