LOCUS       sequence-280199-        1766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0773
FEATURES             Location/Qualifiers
     source          1..1766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggaccact gacatttagg gatgtgaaaa tagaattctc tctagaggaa
       61 tggcaatgcc tggacactgc gcagcggaat ttatatagag atgtgatgtt agagaactac
      121 agaaacctgg tcttccttgt tatgtgttct cattttgccc aagatgtttg gccagagcac
      181 agcataaaag attctttcca aaaagtgata ctgagaacat atggaaaata tggacatgag
      241 aatttacagc taagaaaaga ccataaaagt gtggatgcat gtaaggtgta caaaggaggt
      301 tataatggac ttaaccagtg tttgacaact actgacagca agatatttca gtgtgataaa
      361 tatgtgaaag tctttcataa atttccaaat gtaaatagaa ataagataag acatactgga
      421 aagaaacctt tcaaatgtaa aaaccgtggc aaatcatttt gcatgctttc acaattaact
      481 caacataaga aaattcatac tagagagtat tcttacaaat gtgaagaatg tggtaaagcc
      541 tttaactggt cctcaaccct tactaaacat aagataattc atactggaga aaaaccctac
      601 aaatgtgaag aatgtggcaa agcttttaac cggtcctcaa atcttactaa acataaaata
      661 attcatactg gagagaaacc ctacaaatgt gaagaatgtg gcaaagcttt taaccggtcc
      721 tcaaccctta ctaaacataa aagaattcat acagaagaga aaccctacaa atgtgaagaa
      781 tgtggcaagg cctttaacca gttctcgatt cttaataaac ataagagaat tcatatggaa
      841 gataaaccct acaaatgtga agaatgtggc aaagccttta gagtattctc aattcttaaa
      901 aaacataaga taatccatac tggggaaaaa ccatacaaat gtgaagaatg tggcaaagcc
      961 tttaaccagt tctcaaacct tactaaacat aagataattc atactggaga gaaaccctac
     1021 aaatgtgatg aatgtggcaa agcctttaac cagtcctcaa cccttactaa acataaaaga
     1081 attcatacgg gagaaaaacc ctacaaatgt gaagaatgtg gcaaagcttt taaacagtcc
     1141 tcaaccctta ctgaacataa gataattcat actggagaga aaccctacaa atgtgaaaaa
     1201 tgtggcaagg cctttagctg gtcctcagct tttactaaac ataagagaaa tcatatggaa
     1261 gataaaccct acaaatgtga agaatgtggc aaagccttta gtgtattctc aacccttact
     1321 aaacataaaa taattcatac tagagaaaaa ccctacaaat gtgaagaatg tggcaaagcc
     1381 tttaaccagt cctcaatttt tactaaacat aagataattc acactgaagg gaaatcctac
     1441 aaatgtgaaa aatgtggcaa tgcttttaac cagtcctcaa accttactgc acgtaagata
     1501 atttatactg gagagaaacc ctacaaatat gaagaatgtg acaaagcctt taacaagttc
     1561 tcaaccctta ttacacatca gataatttat actggagaga aaccctgcaa acatgaatgt
     1621 ggcagagcct ttaacaaatc ctcaaattat actaaagaga aactacaaac ctagtaatga
     1681 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1741 gactcagtat tgggctaagt tgggtg
//
