LOCUS       sequence-280205-        1655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0775
FEATURES             Location/Qualifiers
     source          1..1655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtagacaa aaccccagtt atgtgttctc attttgccca agatgtttgg
       61 ccagagcaca gcataaaaga ttctttccaa aaagtgatac tgagaacata tggaaaatat
      121 ggacatgaga atttacagct aagaaaagac cataaaagtg tggatgcatg taaggtgtac
      181 aaaggaggtt ataatggact taaccagtgt ttgacaacta ctgacagcaa gatatttcag
      241 tgtgataaat atgtgaaagt ctttcataaa tttccaaatg taaatagaaa taagataaga
      301 catactggaa agaaaccttt caaatgtaaa aaccgtggca aatcattttg catgctttca
      361 caattaactc aacataagaa aattcatact agagagtatt cttacaaatg tgaagaatgt
      421 ggtaaagcct ttaactggtc ctcaaccctt actaaacata agataattca tactggagaa
      481 aaaccctaca aatgtgaaga atgtggcaaa gcttttaacc ggtcctcaaa tcttactaaa
      541 cataaaataa ttcatactgg agagaaaccc tacaaatgtg aagaatgtgg caaagctttt
      601 aaccggtcct caacccttac taaacataaa agaattcata cagaagagaa accctacaaa
      661 tgtgaagaat gtggcaaggc ctttaaccag ttctcgattc ttaataaaca taagagaatt
      721 catatggaag ataaacccta caaatgtgaa gaatgtggca aagcctttag agtattctca
      781 attcttaaaa aacataagat aatccatact ggggaaaaac catacaaatg tgaagaatgt
      841 ggcaaagcct ttaaccagtt ctcaaacctt actaaacata agataattca tactggagag
      901 aaaccctaca aatgtgatga atgtggcaaa gcctttaacc agtcctcaac ccttactaaa
      961 cataaaagaa ttcatacggg agaaaaaccc tacaaatgtg aagaatgtgg caaagctttt
     1021 aaacagtcct caacccttac tgaacataag ataattcata ctggagagaa accctacaaa
     1081 tgtgaaaaat gtggcaaggc ctttagctgg tcctcagctt ttactaaaca taagagaaat
     1141 catatggaag ataaacccta caaatgtgaa gaatgtggca aagcctttag tgtattctca
     1201 acccttacta aacataaaat aattcatact agagaaaaac cctacaaatg tgaagaatgt
     1261 ggcaaagcct ttaaccagtc ctcaattttt actaaacata agataattca cactgaaggg
     1321 aaatcctaca aatgtgaaaa atgtggcaat gcttttaacc agtcctcaaa ccttactgca
     1381 cgtaagataa tttatactgg agagaaaccc tacaaatatg aagaatgtga caaagccttt
     1441 aacaagttct caacccttat tacacatcag ataatttata ctggagagaa accctgcaaa
     1501 catgaatgtg gcagagcctt taacaaatcc tcaaattata ctaaagagaa actacaaacc
     1561 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1621 ggaaaggacg accagctcgg cctttaggat cactt
//
