LOCUS       sequence-280211-        1976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0787
FEATURES             Location/Qualifiers
     source          1..1976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggggatagg ggtgtcttta ttactgcagt tttctctaac acctgggggc
       61 taccggagtg tgggccgaag caggcgctgc agccgcggaa gtatccccag gaacatcccc
      121 aagaggagct ggaaaaagcc tcatccccag ctctgcagtc tccaggcaga ggaagaaccg
      181 atgctggaac gtcgttgcag gggccccctg gccatgggcc tggcccagcc ccgactcctt
      241 tctgggccct cccaggagtc accccagacc ctggggaagg agtcccgcgg gctgaggcaa
      301 caaggcacgt cagtggccca gtctggtgcc caagccccag gcagggccca tcgctgtgcc
      361 cactgtcgaa ggcacttccc tggctgggtg gctctgtggc ttcacacccg ccggtgccag
      421 gcccggctgc ccttgccctg ccctgagtgt ggccgtcgct ttcgccatgc ccccttctta
      481 gcactgcacc gccaggtcca tgctgctgcc accccagacc tgggctttgc ctgccacctc
      541 tgtgggcaga gcttccgagg ctgggtggcc ctggttctgc atctgcgggc ccattcagct
      601 gcaaagcggc ccatcgcttg tcccaaatgc gagagacgct tctggcgacg aaagcagctt
      661 cgagctcatc tgcggcggtg ccaccctccc gccccggagg cccggccctt catatgcggc
      721 aactgtggcc ggagctttgc ccagtgggac cagctagttg cccacaagcg ggtgcacgta
      781 gctgaggccc tggaggaggc cgcagccaag gctctggggc cccggcccag gggccgcccc
      841 gcggtgaccg ccccccggcc cggtggagat gccgtcgacc gccccttcca gtgtgcctgt
      901 tgtggcaagc gcttccggca caagcccaac ttgatcgctc accgccgcgt gcacacgggc
      961 gagcggcccc accagtgccc cgagtgcggg aagcgcttta ccaataagcc ctatctgact
     1021 tcgcaccggc gcatccacac cggcgagaag ccctacccgt gcaaagagtg cggccgccgc
     1081 ttccggcaca aacccaacct gctgtctcac agcaagattc acaagcgatc cgaggggtcg
     1141 gcccaggccg cccccggccc ggggagcccc cagctgccag ccggccccca ggagtccgcg
     1201 gccgagccca ccccggcggt acctctgaaa ccggcccagg agccgccgcc aggggccccg
     1261 ccagagcacc cgcaggaccc gatcgaagcc cccccctccc tctacagctg cgacgactgc
     1321 ggcaggagct tccggctgga gcgcttcctg cgggcccacc agcggcagca caccggggag
     1381 cggcccttca cctgcgccga gtgcgggaag aacttcggca agaagacgca cctggtggcg
     1441 cactcgcgcg tgcactccgg cgagcggccc ttcgcctgcg aggagtgcgg ccgccgcttc
     1501 tcccagggca gccatctggc ggcgcatcgg cgcgaccacg cccccgatcg gcccttcgtg
     1561 tgtcccgact gcggcaaggc cttccgccac aaaccctacc tggcggcgca ccggcgcatc
     1621 cacaccggcg agaagcccta cgtctgcccc gactgcggca aagccttcag ccagaagtcc
     1681 aacctggtgt cgcaccggcg catccacacg ggcgagcggc cctacgcctg tcccgactgc
     1741 gaccgcagct tcagccagaa gtccaacctc atcacccacc gcaagagcca catccgggac
     1801 ggcgccttct gctgtgccat ctgtggccag accttcgacg acgaggagag actcctggcc
     1861 caccagaaga agcacgatgt ctagtaatga actagtccgt aacttgaaag tatttcgatt
     1921 tcttggcttt atatatcttg tggaaaggac gatctgggcg ccataacaca cgtgtc
//
