LOCUS       sequence-280217-        3419 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0808
FEATURES             Location/Qualifiers
     source          1..3419
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgcagc ggcgggcggc ggcgggccgg ggacagcggt aggcgccacg
       61 ggctcgggga ttgcggcggc agccgcaggc ctagctgttt atcgacggaa ggatgggggc
      121 ccggccacca agttttggga gagcccggag acggtgtccc agctggattc ggtgcgggtc
      181 tggctgggca agcactacaa gaagtatgtt catgcggatg ctcctaccaa taaaacactg
      241 gctgggctgg tggtgcagct tcttcagttc caggaagatg cctttgggaa gcatgtcacc
      301 aacccggcct tcaccaaact ccctgcaaag tgtttcatgg atttcaaagc tggaggcgcc
      361 ttatgtcaca ttcttggggc tgcttacaag tataaaaatg aacagggatg gcggaggttt
      421 gacctacaga acccatctcg aatggatcgt aatgtggaaa tgtttatgaa cattgaaaaa
      481 acattggtgc agaacaattg tttgaccaga cccaacatct acctcattcc agacattgat
      541 ctgaagttgg ctaacaaatt gaaagatatc atcaaacgac atcagggaac atttacggat
      601 gagaagtcaa aagcttccca ccacatttac ccatattctt cctcacaaga cgatgaagaa
      661 tggttgagac cggtgatgag aaaagagaag caagtgttag tgcattgggg cttttaccca
      721 gacagctatg atacttgggt ccatagtaat gatgttgatg ctgaaattga agatccacca
      781 attccagaaa aaccatggaa ggttcatgtg aaatggattt tggacactga tattttcaat
      841 gaatggatga atgaggagga ttatgaggtg gatgaaaata ggaagcctgt gagttttcgt
      901 cagcggattt caaccaagaa tgaagagcca gtcagaagtc cagaaagaag agatagaaaa
      961 gcatcagcta atgctcgaaa gaggaaacat tcgccttcgc ctccccctcc gacaccaaca
     1021 gaatcacgga agaagagtgg gaagaaaggc caagctagcc tttatgggaa gcgcagaagt
     1081 cagaaagagg aagatgagca agaagatcta accaaggata tggaagaccc aacacctgta
     1141 cccaatatag aagaagtagt acttcccaaa aatgtgaacc taaagaaaga tagtgaaaat
     1201 acacctgtta aaggaggaac tgtagcggat ctagatgagc aggatgaaga aacagtcaca
     1261 gcaggaggaa aggaagatga agatcctgcc aaaggtgatc agagtcgatc agttgacctt
     1321 ggggaagata atgtgacaga gcagaccaat cacattatta ttcctagtta tgcatcatgg
     1381 tttgattata actgtattca tgtgattgaa cggcgtgctc ttcctgagtt cttcaatgga
     1441 aaaaacaaat ccaagactcc agaaatatac ttggcatatc gaaattttat gattgacacg
     1501 tatcgtctaa acccccaaga gtatttaact agcactgctt gtcggaggaa cttgactgga
     1561 gatgtgtgtg ctgtgatgag ggtccatgcc tttttagagc agtggggact cgttaattac
     1621 caagttgacc cggaaagtag acccatggca atgggacctc ctcctactcc tcattttaat
     1681 gtattagctg ataccccctc tgggcttgtg cctctgcatc ttcgatcacc tcaggttcct
     1741 gctgctcaac agatgctaaa ttttcctgag aaaaacaagg aaaaaccagt tgatttgcag
     1801 aactttggtc tccgtactga catttactcc aagaaaacat tagcaaagag taaaggtgct
     1861 agtgctggaa gagaatggac tgaacaggag acccttctac tcctggaggc cctggagatg
     1921 tacaaggatg attggaacaa agtgtcggaa catgttggaa gtcgtactca ggatgaatgc
     1981 atcctccact ttttgagact tcccattgag gacccatacc ttgagaattc agatgcttcc
     2041 cttgggcctt tggcctacca gcctgtcccc ttcagtcagt caggaaatcc agttatgagt
     2101 actgttgctt ttttggcatc tgtggtggac cctcgcgtgg catctgctgc agcaaaagcg
     2161 gctttggagg agttttctcg ggtccgggag gaggtaccac tggaattggt tgaagctcat
     2221 gtcaagaaag tacaagaagc agcacgagcc tctgggaaag tggatcccac ctacggtctg
     2281 gagagcagct gcattgcagg cacagggccc gatgagccag agaagcttga aggagctgaa
     2341 gaggaaaaaa tggaagccga ccctgatggt cagcagcctg aaaaggcaga aaataaagtg
     2401 gaaaatgaaa cggatgaagg tgataaagca caagatggag aaaatgaaaa aaatagtgaa
     2461 aaggaacagg atagtgaagt gagtgaggat accaaatcag aagaaaagga gactgaagag
     2521 aacaaagaac tcactgatac atgtaaagaa agagaaagtg atactgggaa gaagaaagta
     2581 gaacatgaaa tttccgaagg aaatgttgcc acagccgcag cagctgctct tgcctcagcg
     2641 gctaccaaag ccaagcacct ggctgcagtg gaagaaagaa agatcaagtc cctggtagct
     2701 ctcttggttg agacacaaat gaagaaacta gagatcaaac ttcgacattt tgaagagctg
     2761 gaaactatca tggacagaga gaaagaagct ctagaacaac agaggcagca gttgcttact
     2821 gaacgccaaa acttccacat ggaacagctg aagtatgctg aattacgagc acgacagcaa
     2881 atggaacagc agcagcatgg ccagaaccct caacaggcac accagcactc aggaggacct
     2941 ggcctggccc cacttggagc agcagggcac cctggcatga tgcctcatca acagccccct
     3001 ccctaccctc tgatgcacca ccagatgcca ccacctcatc caccccagcc aggtcagata
     3061 ccaggcccag gttccatgat gcccgggcag cacatgccag gccgcatgat tcccactgtt
     3121 gcagccaaca tccacccctc tgggagtggc cctacccctc ctggcatgcc accaatgcca
     3181 ggaaacatct taggaccccg ggtacccctg acagcaccta acggcatgta tccccctcca
     3241 ccacagcagc agccaccgcc accaccacct gcagatgggg tccctccgcc tcctgctcct
     3301 ggcccgccag cctcagctgc tccttagtaa tgaactagtc cgtaacttga aagtatttcg
     3361 atttcttggc tttatatatc ttgtggaaag gacgaggtta atgaagtcgc tgggtcccg
//
