LOCUS       sequence-280226-        1634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0836
FEATURES             Location/Qualifiers
     source          1..1634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1311..1320
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgttgccttt ggagaaggcg tttgcctccc ccaggagctc cccagccccg
       61 ccggatctgc ccacgccggg gtcagcagcc ggagtccagc aggaagaacc cgagaccatc
      121 cctgagagga cccctgctga cctggagttc tcccgcctgc gtttccggga atttgtctac
      181 caggaggctg ccgggcccca ccagaccctg gcccggctgc atgagctgtg ccgccagtgg
      241 ctgatgcctg aggcgcgctc caaggagcag atgctggagc tgctggtgct ggagcagttc
      301 ctgggcatcc tgcctgataa ggtccggccc tgggtggtgg cacagtaccc tgagagctgc
      361 aagaaggcag cctccctggt ggagggcctc gctgatgtcc tggaagagcc agggatgctg
      421 ctgggctccc ctgcgggctc atcctcaatt cttagcgatg gagtgtacga gaggcacatg
      481 gaccctctgc tgctaccagg cgagctcgcg agccccagcc aggcccttgg agctggggag
      541 atcccggcac cttctgagac accctggctt tctccggacc ccctgtttct ggaacagagg
      601 agggtcagag aagcaaagac cgaagaggac ggccctgcca acaccgagca gaagctgaag
      661 tcctttccag aggaccctca gcacctgggg gagtggggcc acctggaccc tgccgaggag
      721 aacctgaaga gctaccggaa gctgctcctg tgggggtatc agctttccca gcctgacgct
      781 gcctccaggc tggacactga ggaactccgg ttggtggaaa gagatccaca aggaagcagc
      841 ctcccagaag gcgggaggcg gcaggagagc gctgggtgcg cctgcgagga ggccgccccc
      901 gcgggggtgc tgcctgagct gcctacggag gcgccccctg gggacgccct tgccgatccc
      961 ccgtcgggca ccactgagga ggaggaagag cagcctggga aggccccgga cccgcaggac
     1021 ccccaggacg cggagtccga ctctgccacc ggatcgcaga ggcagtccgt catccagcag
     1081 cctgccccgg acaggggcac ggcgaaactg ggaaccaaga ggccgcaccc cgaggatggg
     1141 gacgggcaga gcctcgaggg cgtctctagc tccggcgaca gcgcagggct ggaggccggg
     1201 cagggccctg gggctgacga gccgggcttg tcccgcggga agccctatgc ctgcggcgag
     1261 tgcggggagg ccttcgcgtg gctctcgcac ctgatggagc accacagcag ccatggcggc
     1321 cggaagcgct acgcctgtca gggctgctgg aagaccttcc acttcagcct ggccctagcc
     1381 gagcaccaga agacccacga gaaggagaaa agctacgcgc tggggggcgc ccggggcccc
     1441 caaccgtcca cccgcgaagc ccaggcgggg gctagggcgg gcggtccccc agagagcgtg
     1501 gagggcgagg ctccccccgc acccccagag gcgcagaggt agtaatgaac tagtccgtaa
     1561 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga agcgcaccgg
     1621 gcaaacttca taca
//
