LOCUS       sequence-280232-        1802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0838
FEATURES             Location/Qualifiers
     source          1..1802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1479..1488
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgcggagagt cggagggcga gctgacgagg acgctacggc ggcgggaagt
       61 ctgtgggttc tggcaccacc tgagcccact gggcatctgg tcatccctgg cacctctcct
      121 ttggagccac cttgtccctg gctagacagt cacattttcc agtgccgttt tggaaagatg
      181 ttgcctttgg agaaggcgtt tgcctccccc aggagctccc cagccccgcc ggatctgccc
      241 acgccggggt cagcagccgg agtccagcag gaagaacccg agaccatccc tgagaggacc
      301 cctgctgacc tggagttctc ccgcctgcgt ttccgggaat ttgtctacca ggaggctgcc
      361 gggccccacc agaccctggc ccggctgcat gagctgtgcc gccagtggct gatgcctgag
      421 gcgcgctcca aggagcagat gctggagctg ctggtgctgg agcagttcct gggcatcctg
      481 cctgataagg tccggccctg ggtggtggca cagtaccctg agagctgcaa gaaggcagcc
      541 tccctggtgg agggcctcgc tgatgtcctg gaagagccag ggatgctgct gggctcccct
      601 gcgggctcat cctcaattct tagcgatgga gtgtacgaga ggcacatgga ccctctgctg
      661 ctaccaggcg agctcgcgag ccccagccag gcccttggag ctggggagat cccggcacct
      721 tctgagacac cctggctttc tccggacccc ctgtttctgg aacagaggag ggtcagagaa
      781 gcaaagaccg aagaggacgg ccctgccaac accgagcaga agctgaagtc ctttccagag
      841 gaccctcagc acctggggga gtggggccac ctggaccctg ccgaggagaa cctgaagagc
      901 taccggaagc tgctcctgtg ggggtatcag ctttcccagc ctgacgctgc ctccaggctg
      961 gacactgagg aactccggtt ggtggaaaga gatccacaag gaagcagcct cccagaaggc
     1021 gggaggcggc aggagagcgc tgggtgcgcc tgcgaggagg ccgcccccgc gggggtgctg
     1081 cctgagctgc ctacggaggc gccccctggg gacgcccttg ccgatccccc gtcgggcacc
     1141 actgaggagg aggaagagca gcctgggaag gccccggacc cgcaggaccc ccaggacgcg
     1201 gagtccgact ctgccaccgg atcgcagagg cagtccgtca tccagcagcc tgccccggac
     1261 aggggcacgg cgaaactggg aaccaagagg ccgcaccccg aggatgggga cgggcagagc
     1321 ctcgagggcg tctctagctc cggcgacagc gcagggctgg aggccgggca gggccctggg
     1381 gctgacgagc cgggcttgtc ccgcgggaag ccctatgcct gcggcgagtg cggggaggcc
     1441 ttcgcgtggc tctcgcacct gatggagcac cacagcagcc atggcggccg gaagcgctac
     1501 gcctgtcagg gctgctggaa gaccttccac ttcagcctgg ccctagccga gcaccagaag
     1561 acccacgaga aggagaaaag ctacgcgctg gggggcgccc ggggccccca accgtccacc
     1621 cgcgaagccc aggcgggggc tagggcgggc ggtcccccag agagcgtgga gggcgaggct
     1681 ccccccgcac ccccagaggc gcagaggtag taatgaacta gtccgtaact tgaaagtatt
     1741 tcgatttctt ggctttatat atcttgtgga aaggacgact gtattaagcg tgcagcaatg
     1801 gg
//
