LOCUS       sequence-280235-        1379 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0860
FEATURES             Location/Qualifiers
     source          1..1379
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggatcaaa aatgaatctc attgaacatt cccatttacc taccacagat
       61 gaattttctt tttctgaaaa tttatttggt gttttaacag aacaagtggc aggtcctctg
      121 ggacagaacc tggaagtgga accatactcg caatacagca atgttcagtt tccccaagtt
      181 caaccacaga tttcctcgtc atcctattat tccaacctgg gtttctaccc ccagcagcct
      241 gaagagtggt actctcctgg aatatatgaa ctcaggcgta tgccagctga gactctctac
      301 cagggagaaa ctgaggtagc agagatgcct gtaacaaaga agccccgcat gggcgcgtca
      361 gcagggagga tcaaagggga tgagctgtgt gttgtttgtg gagacagagc ctctggatac
      421 cactataatg cactgacctg tgaggggtgt aaaggcttgt taactgaaat tcagtgtaaa
      481 tctaagcgac tgagaaaaaa tgtgaagcag catgcagatc agaccgtgaa tgaagacagt
      541 gaaggtcgtg acttgcgaca agtgacctcg acaacaaagt catgcaggga gaaaactgaa
      601 ctcaccccag atcaacagac tcttctacat tttattatgg attcatataa caaacagagg
      661 atgcctcagg aaataacaaa taaaatttta aaagaagaat tcagtgcaga agaaaatttt
      721 ctcattttga cggaaatggc aaccaatcat gtacaggttc ttgtagaatt cacaaaaaag
      781 ctaccaggat ttcagacttt ggaccatgaa gaccagattg ctttgctgaa agggtctgcg
      841 gttgaagcta tgttccttcg ttcagctgag attttcaata agaaacttcc gtctgggcat
      901 tctgacctat tggaagaaag aattcgaaat agtggtatct ctgatgaata tataacacct
      961 atgtttagtt tttataaaag tattggggaa ctgaaaatga ctcaagagga gtatgctctg
     1021 cttacagcaa ttgttatcct gtctccagat agacaataca taaaggatag agaggcagta
     1081 gagaagcttc aggagccact tcttgatgtg ctacaaaagt tgtgtaagat tcaccagcct
     1141 gaaaatcctc aacactttgc ctgtctcctg ggtcgcctga ctgaattacg gacattcaat
     1201 catcaccacg ctgagatgct gatgtcatgg agagtaaacg accacaagtt taccccactt
     1261 ctctgtgaaa tctgggacgt gcagtagtaa tgaactagtc cgtaacttga aagtatttcg
     1321 atttcttggc tttatatatc ttgtggaaag gacgaatgtt ccgagaccac cgtgcacga
//
