LOCUS       sequence-28025-e         605 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova M, Dekelver RC, 
            Katibah GE, Amora R, Boydston EA, Zeitler B, Meng X, Miller JC, 
            Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R
  TITLE     Efficient targeting of expressed and silent genes in human ESCs and 
            iPSCs using zinc-finger nucleases.
  JOURNAL   Nat Biotechnol. 2009 Sep;27(9):851-7. doi: 10.1038/nbt.1562
  PUBMED    19680244
REFERENCE   2  (bases 1 to 605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2009-09"; volume: "27"; issue:
            "9"; pages: "851-7"
COMMENT     Sequence Label: TRE-TIGHT-EGFP-backward donor
FEATURES             Location/Qualifiers
     source          1..605
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    158..239
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(195..214)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 angcgtgcta gcgcggccgc atcgataagc ttgtcnacna tatctctaga ggatcataat
       61 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
      121 gaacctgaaa cataaaatga atgcnnntgt tgttgttaac ttgtttattg cagcttataa
      181 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgcc
      241 tcnacccata gagcccaccg catccccagc atgcctgcta ttgtcttccc aatcctcccc
      301 cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga
      361 tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga
      421 aggcacgggg gaggggcaaa caacagatgg ctggcaacta naangcacnn tcnaggctga
      481 tcagcgngnt tnaacgggcc ctctagatca ngcaccgggc ttgcgggtca tgcaccangt
      541 gcgcggtcct tnnnggcacc tcgacgtcng cggtgacggt gaagccgagc cgctcgtaga
      601 angng
//
