LOCUS       sequence-280250-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0890
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgcggg gatgtatttg gaacattatc tggacaacct gaaggctgaa
       61 attgacaagt tggccactga gtatatgagt agtgcccgca gcctgagctc cgaggaaaaa
      121 ttggcccttc tcaaacagat ccaggaagcc tatggcaagt gcaaggaatt tggtgacgac
      181 aaggtgcagc ttgccatgca gacctatgag atggtggaca aacacattcg gcggctggac
      241 acagacctgg cccgttttga ggctgatctc aaggagaaac agattgagtc aagtgactat
      301 gacagctctt ccagcaaagg caaaaagaaa ggccggactc aaaaggagaa gaaagctgct
      361 cgtgctcgtt ccaaagggaa aaactcggat gaagaagccc ccaagactgc ccagaagaag
      421 ttaaagctcg tgcgcacaag tcctgagtat gggatgccct cagtgacctt tggcagtgtc
      481 cacccctctg atgtgttgga tatgcctgtg gatcccaacg aacccaccta ttgcctttgt
      541 caccaggtct cctatggaga gatgattggc tgtgacaacc ctgattgttc cattgagtgg
      601 ttccattttg cctgtgtggg gctgacaacc aagcctcggg ggaaatggtt ttgcccacgc
      661 tgctcccaag aacggaagaa gaaatagtaa tgaactagtc cgtaacttga aagtatttcg
      721 atttcttggc tttatatatc ttgtggaaag gacgaatgca cagcgttagg atgctttgt
//
