LOCUS       sequence-280253-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0892
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgcggg gatgtatttg gaacattatc tggacagtat tgaaaacctt
       61 ccctttgaat tacagagaaa ctttcagctc atgagggacc tagaccaaag aacagaggac
      121 ctgaaggctg aaattgacaa gttggccact gagtatatga gtagtgcccg cagcctgagc
      181 tccgaggaaa aattggccct tctcaaacag atccaggaag cctatggcaa gtgcaaggaa
      241 tttggtgacg acaaggtgca gcttgccatg cagacctatg agatggtgga caaacacatt
      301 cggcggctgg acacagacct ggcccgtttt gaggctgatc tcaaggagaa acagattgag
      361 tcaagtgact atgacagctc ttccagcaaa ggccggactc aaaaggagaa gaaagctgct
      421 cgtgctcgtt ccaaagggaa aaactcggat gaagaagccc ccaagactgc ccagaagaag
      481 ttaaagctcg tgcgcacaag tcctgagtat gggatgccct cagtgacctt tggcagtgtc
      541 cacccctctg atgtgttgga tatgcctgtg gatcccaacg aacccaccta ttgcctttgt
      601 caccaggtct cctatggaga gatgattggc tgtgacaacc ctgattgttc cattgagtgg
      661 ttccattttg cctgtgtggg gctgacaacc aagcctcggg ggaaatggtt ttgcccacgc
      721 tgctcccaag aacggaagaa gaaatagtaa tgaactagtc cgtaacttga aagtatttcg
      781 atttcttggc tttatatatc ttgtggaaag gacgaaacaa attatgcgct tccaccagg
//
