LOCUS       sequence-280259-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0897
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagatcct gaaggagcta gacgagtgct acgagcgctt cagtcgcgag
       61 acagacgggg cgcagaagcg gcggatgctg cactgtgtgc agcgcgcgct gatccgcagc
      121 caggagctgg gcgacgagaa gatccagatc gtgagccaga tggtggagct ggtggagaac
      181 cgcacgcggc aggtggacag ccacgtggag ctgttcgagg cgcagcagga gctgggcgac
      241 acagcgggca acagcggcaa ggctggcgcg gacaggccca aaggcgaggc ggcagcgcag
      301 gctgacaagc ccaacagcaa gcgctcacgg cggcagcgca acaacgagaa ccgtgagaac
      361 gcgtccagca accacgacca cgacgacggc gcctcgggca cacccaagga gaagaaggcc
      421 aagacctcca agaagaagaa gcgctccaag gccaaggcgg agcgagaggc gtcccctgcc
      481 gacctcccca tcgaccccaa cgaacccacg tactgtctgt gcaaccaggt ctcctatggg
      541 gagatgatcg gctgcgacaa cgacgagtgc cccatcgagt ggttccactt ctcgtgcgtg
      601 gggctcaatc ataaacccaa gggcaagtgg tactgtccca agtgccgggg ggagaacgag
      661 aagaccatgg acaaagccct ggagaaatcc aaaaaagaga gggcttacaa caggtagtaa
      721 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
      781 gacgatgaac caaacgtaga agccatgtg
//
