LOCUS       sequence-28027-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova M, Dekelver RC, 
            Katibah GE, Amora R, Boydston EA, Zeitler B, Meng X, Miller JC, 
            Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R
  TITLE     Efficient targeting of expressed and silent genes in human ESCs and 
            iPSCs using zinc-finger nucleases.
  JOURNAL   Nat Biotechnol. 2009 Sep;27(9):851-7. doi: 10.1038/nbt.1562
  PUBMED    19680244
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2009-09"; volume: "27"; issue:
            "9"; pages: "851-7"
COMMENT     Sequence Label: TV-hPITX3-Reverse
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    41..74
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 acccggtaga atttcgaggt cgagatccgg aacccttaat ataacttcnt ataatgtatg
       61 ctatacgaag ttattaggtc cctcgaagag gttcactagt actggccatt gcggccatcc
      121 tgcaagggcc aggagcacag cggtaagcgc gcccccttcc gggggtgcag gacataacag
      181 cttcatcccc agtgcgggtg ggaccccggc tgggcagccc cggtgggagc cagcgagtgg
      241 cttaggaggt ccccgagccg gagaatatgc gctggcttgg gcgctctgtg acctgccccc
      301 accctggccc ccagactcag aaaaggcctc ggcttcgctg cccggcggct ccccagagga
      361 cggttcgctg aaaaagaagc agcggcggca gcgcacgcac ttcaccagcc agcagctaca
      421 ggagctagag gcgaccttcc agaggaaccg ctaccccgac atgagcacgc gcgaggagat
      481 cgccgtgtgg accaacctca ccgaggcccg cgtgcgggta tgctctccag acccgcgact
      541 cgcacccgcg cgggccctcc gcgctcagcc tgcgacccgg aggccagcgg ttccacccca
      601 cctgccccct ccctcggacc ccggaccccg gaccctggct cccggccgca gccccacgcg
      661 gtctcttgcc cgccannctc cancctctgc tcccnctgct ccctactact cggancgcgc
      721 gcttcttcgg ncctgacacc gtct
//
