LOCUS       sequence-280280-        1274 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0977
FEATURES             Location/Qualifiers
     source          1..1274
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagaaat ccacaatgga ggggaactct gtgactttat ggaaaatgga
       61 gaaatcttca gtgaacactc atgccttaat gcacacatgg gaactgaaaa tacaggggac
      121 acttatgact gtgatgagta tggagaaaac tttcccatgt tacacaacag tgcccctgct
      181 ggagagacac tttctgtgtt gaatcagtgc agaaaagcct tcagcctgcc accaaatgtt
      241 caccagagaa cgtggatagg agacaaatcc tttgaataca gtgactgtga ggaagccttt
      301 gttgatcagt cacatcttca ggcaaatagg ataactcaca atggagaaac actctatgaa
      361 cagaagcaat gtgggagagc ttttacttac tccacaagcc atgctgtgtc tgttaaaatg
      421 catactgtag aaaaacccta cgaatgtaag gaatgtggaa aattctttag atattcttca
      481 tatcttaata gtcacatgcg aacccatact ggggagaaac cctatgaatg taaggaatgt
      541 gggaaatgct tcactgtttc ttcacaccta gttgaacatg taagaattca tactggagag
      601 aaaccttatc aatgtaagga atgtggaaga gccttcgctg ggcgctcagg ccttactaaa
      661 catgtacgaa tacacactgg agagaagccc tatgaatgta acgaatgtgg gaaagcctac
      721 aataggtttt atctactaac tgaacatttt aaaactcaca cagaggagaa gccctttgaa
      781 tgtaaggtat gtggaaaatc cttcagaagc tcttcatgcc ttaagaatca ctttagaatt
      841 cacactggaa taaaacccta taaatgtaag gagtgtggga aagcattcac tgtttcctca
      901 agcttacata atcatgtaaa aatccatact ggagagaagc cctatgaatg taaggactgt
      961 gggaaagcct ttgctacatc ttcacaactc attgaacata taagaactca cactggagag
     1021 aaaccgtata tatgtaagga atgtgggaaa accttccgtg cttcttcaca tctacagaaa
     1081 catgttagaa ttcacactgg agagaaaccc tatatatgta acgaatgtgg gaaagcctac
     1141 aatagatttt atttacttac taaacattta aaaacacact agtaatgaac tagtccgtaa
     1201 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga gtagtaacaa
     1261 cccataagtt gctg
//
