LOCUS       sequence-280295-        1250 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1032
FEATURES             Location/Qualifiers
     source          1..1250
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtgcccag ctcgccctcg ccccctccgc ctcctcgggt ctacaagcca
       61 tgcttcgtgt gcaatgacaa gtcctctggc taccactatg gggtcagctc ttgtgaaggc
      121 tgcaagggct tctttcgccg aagcatccag aagaacatgg tgtacacgtg tcaccgcgac
      181 aaaaactgta tcatcaacaa ggtgaccagg aatcgctgcc agtactgccg gctacagaag
      241 tgcttcgaag tgggcatgtc caaggaagct gtgcgaaatg accggaacaa gaagaagaaa
      301 gaggtgaagg aagaagggtc acctgacagc tatgagctga gccctcagtt agaagagctc
      361 atcaccaagg tcagcaaagc ccatcaggag actttcccct cgctctgcca gctgggcaag
      421 tataccacga actccagtgc agaccaccgc gtgcagctgg atctggggct gtgggacaag
      481 ttcagtgagc tggctaccaa gtgcatcatc aagatcgtgg agtttgccaa gcggttgcct
      541 ggctttacag ggctcagcat tgctgaccag atcactctgc tcaaagctgc ctgcctagat
      601 atcctgatgc tgcgtatctg cacaaggtac accccagagc aggacaccat gaccttctcc
      661 gacgggctga ccctgaaccg gacccagatg cacaatgccg gcttcgggcc cctcacagac
      721 cttgtctttg cctttgctgg gcagctcctg cccctggaga tggatgacac cgagacaggg
      781 ctgctcagcg ccatctgcct catctgcgga gaccgcatgg acctggagga gcccgaaaaa
      841 gtggacaagc tgcaggagcc actgctggaa gccctgaggc tgtacgcccg gcgccggcgg
      901 cccagccagc cctacatgtt cccaaggatg ctaatgaaaa tcaccgacct ccggggcatc
      961 agcactaagg gagctgaaag ggccattact ctgaagatgg agattccagg cccgatgcct
     1021 cccttaatcc gagagatgct ggagaaccct gaaatgtttg aggatgactc ctcgcagcct
     1081 ggtccccacc ccaatgcctc tagcgaggat gaggttcctg ggggccaggg caaagggggc
     1141 ctgaagtccc cagcctagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1201 ctttatatat cttgtggaaa ggacgactgc ggccataatg tttcctatgg
//
