LOCUS       sequence-280298-        1493 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1054
FEATURES             Location/Qualifiers
     source          1..1493
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccacgtt cccctgccag ttatgtggca agacgttcct caccctggag
       61 aagttcacga ttcacaatta ttcccactcc agggagcggc cgtacaagtg tgtgcagcct
      121 gactgtggca aagcctttgt ttccagatat aaattgatga ggcatatggc tacccattct
      181 ccccagaaat ctcaccagtg tgctcactgt gagaagacgt tcaaccggaa agaccacctg
      241 aaaaaccacc tccagaccca cgaccccaac aaaatggcct ttgggtgtga ggagtgtggg
      301 aagaagtaca acaccatgct gggctataag aggcacctgg ccctccatgc ggccagcagt
      361 ggggacctca cctgtggggt ctgtgccctg gagctaggga gcaccgaggt gctactggac
      421 cacctcaaag cccatgcgga agagaagccc cctagcggaa ccaaggaaaa gaagcaccag
      481 tgcgaccact gtgaaagatg cttctacacc cggaaggatg tgcgacgcca cctggtggtc
      541 cacacaggat gcaaggactt cctgtgccag ttctgtgccc agagatttgg gcgcaaggat
      601 cacctcaccc ggcataccaa gaagacccac tcacaggagc tgatgaaaga gagcttgcag
      661 accggagacc ttctgagcac cttccacacc atctcgcctt cattccaact gaaggctgct
      721 gccttgcctc ctttcccttt aggagcttct gcccagaacg ggcttgcaag tagcttgcca
      781 gctgaggtcc atagcctcac cctcagtccc ccagaacaag ccgcccagcc tatgcagccg
      841 ctgccagagt ccctggcctc cctccacccc tcggtatccc ctggctctcc tccgccaccc
      901 cttcccaatc acaagtacaa caccacttct acctcatact ccccacttgc aagcctgccc
      961 ctcaaagcag atactaaagg tttttgcaat atcagtttgt ttgaggactt gcctctgcaa
     1021 gagcctcagt cacctcaaaa gctcaaccca ggttttgatc tggctaaggg aaatgctggt
     1081 aaagtaaacc tgcccaagga gctgcctgca gatgctgtga acctaacaat acctgcctct
     1141 ctggacctgt cccccctgtt gggcttctgg cagctgcccc ctcctgctac ccaaaatacc
     1201 tttgggaata gcactcttgc cctggggcct ggggaatctt tgccccacag gttaagctgt
     1261 ctggggcagc agcagcaaga acccccactt gccatgggca ctgtgagcct gggccagctc
     1321 cccctgcccc ccatccctca tgtgttctca gctggcactg gctctgccat cctgcctcat
     1381 ttccatcatg cattcagata gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1441 tggctttata tatcttgtgg aaaggacgat tccgttaatg tcaagtagaa ggt
//
