LOCUS       sequence-28030-e         471 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova M, Dekelver RC, 
            Katibah GE, Amora R, Boydston EA, Zeitler B, Meng X, Miller JC, 
            Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R
  TITLE     Efficient targeting of expressed and silent genes in human ESCs and 
            iPSCs using zinc-finger nucleases.
  JOURNAL   Nat Biotechnol. 2009 Sep;27(9):851-7. doi: 10.1038/nbt.1562
  PUBMED    19680244
REFERENCE   2  (bases 1 to 471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2009-09"; volume: "27"; issue:
            "9"; pages: "851-7"
COMMENT     Sequence Label: OCT4 GFP puro Donor 2
FEATURES             Location/Qualifiers
     source          1..471
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(82..101)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ttgaattttg accttcttaa gcttgcggga gacgtcgagt ccaaccccgg gcccgcatgc
       61 aagcttcagc tgaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
      121 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
      181 cgccacaccg tcnatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
      241 ctcacgcgcg tcnggntcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
      301 gcggtctgga ncacgccgga nagcgtngan gcgggggcgg tgttcgccga gatcggcccg
      361 cgcatggncn anttgagcgg ttcccggnng gncgcncagc aacagatgga aggnctcctg
      421 gcgccgcacc ggcccaagga ncccgcgtgg ttcctggcca ccgtcngcgt c
//
