LOCUS       sequence-280310-        2036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1082
FEATURES             Location/Qualifiers
     source          1..2036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagacta ttcaacagtg cctcccccct cttctggctc agctggtggc
       61 ggtggtggcg gcggtggtgg tggaggagtt aacgacgctt tcaaagatgc actgcagaga
      121 gcccggcaga ttgcagcaaa aattggaggt gatgcaggga catcactgaa ttcaaatgac
      181 tatggttatg ggggacaaaa aagaccttta gaagatggag atcaaccaga tgctaagaaa
      241 gttgctcctc aaaatgactc ttttggaaca cagttaccac cgatgcatca gcagcaaagc
      301 agatctgtaa tgacagaaga atacaaagtt ccagatggaa tggttggatt cataattggc
      361 agaggaggtg aacagatctc acgcatacaa caggaatctg gatgcaaaat acagatagct
      421 cctgacagtg gtggccttcc agaaaggtcc tgtatgttaa ctggaacacc tgaatctgtc
      481 cagtcagcaa aacggttact ggaccagatt gttgaaaaag gaagaccagc tcctggcttc
      541 catcatggcg atggaccggg aaatgcagtt caagaaatca tgattccagc tagcaaggca
      601 ggattagtca ttggaaaagg gggagaaact attaaacagc ttcaggaacg ggctggagtt
      661 aaaatggtta tgattcaaga cgggccgcag aacactggtg ctgacaaacc tcttaggatt
      721 acaggagacc catataaagt tcaacaagcc aaggaaatgg tgttagagtt aattcgtgat
      781 caaggcggtt tcagagaagt tcggaatgag tatgggtcaa gaataggagg aaatgaaggg
      841 atagatgtcc ccattccaag atttgctgtt ggcattgtaa taggaagaaa tggagagatg
      901 atcaaaaaaa tacaaaatga tgctggtgtt cgcattcagt ttaagccaga tgatgggaca
      961 acacccgaaa ggatagcaca aataacagga cctccagacc gatgtcaaca tgctgcagaa
     1021 attattacag accttcttcg aagtgttcag gctggtaatc ctggtggacc tggacctggt
     1081 ggtcgaggaa gaggtagagg tcaaggcaac tggaacatgg gaccacctgg tggactacag
     1141 gaatttaatt ttattgtgcc aactgggaaa actggattaa taataggaaa aggaggtgaa
     1201 accataaaaa gcataagcca gcagtctggt gcaagaatag aacttcagag aaatcctcca
     1261 ccaaatgcag atcctaatat gaagttattt acaattcgtg gcactccaca acagatagac
     1321 tatgctcggc aactcataga agaaaagatt ggtggcccag taaatccttt agggccacct
     1381 gtaccccatg ggccccatgg tgtcccaggc ccccatggac ctcctgggcc tccagggcct
     1441 ggaactccaa tgggaccata caaccctgca ccttataatc ctggaccacc aggcccggct
     1501 cctcatggtc ctccagcccc atatgctccc cagggatggg gaaatgcata tccacactgg
     1561 cagcagcagg ctcctcctga tccagctaag gcaggaacgg atccaaattc agcagcttgg
     1621 gctgcttatt acgctcacta ttatcaacag caagcacagc caccaccagc agcccctgca
     1681 ggtgcaccaa ctacaactca aactaatgga caaggagatc agcagaatcc agccccagct
     1741 ggacaggttg attataccaa ggcttgggaa gagtactaca agaaaatggg tcaggcagtt
     1801 cctgctccga ctggggctcc tccaggtggt cagccagatt atagtgcagc ctgggctgag
     1861 tattatagac aacaagcagc ctattatgcc cagacaagtc cccagggaat gccacagcat
     1921 cctccagcac ctcagggcca atagtaatga actagtccgt aacttgaaag tatttcgatt
     1981 tcttggcttt atatatcttg tggaaaggac gaaggcacgt agaggatagg tctacc
//
