LOCUS       sequence-280322-        1427 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1092
FEATURES             Location/Qualifiers
     source          1..1427
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagatctc ctctcatcag tctcacctcc tgcagcaact gaacgagcag
       61 cgcaggcaag atgtattttg tgactgcagt attctagttg aagggaaggt cttcaaagca
      121 catcgaaatg tattattcgc tagtagcggc tactttaaaa tgcttctttc tcagaattca
      181 aaggagacga gtcagccaac cacagctaca tttcaggctt tctcccctga cacttttaca
      241 gttatcttgg acttcgtata ttctggcaaa ctgtctctta ctggtcagaa tgtcatagaa
      301 gtgatgtcgg ctgctagctt ccttcagatg actgatgtca taagtgtatg taagactttt
      361 attaaatctt ccttagacat tagtgagaaa gaaaaagatc gctatttcag tctctcagat
      421 aaagatgcca attctaatgg tgtagaacgt tcctcttttt atagtggtgg ctggcaagaa
      481 ggaagcagtt ctccacgttc tcacctaagc ccagagcaag gaacaggtat aataagtgga
      541 aaatcttgga ataagtataa ttatcatcca gcctcccaga agaatactca acaacccttg
      601 gccaagcatg aaccaaggaa agagtccatt aaaaagacca aacatttgag attgtcacag
      661 ccttctgaag ttactcatta taagtcaagc aaacgagaag tacgaacatc tgattcttcc
      721 agccatgttt cccagtctga agaacaagca cagattgatg ctgaaatgga ctctactcct
      781 gttggctatc agtacggtca aggatctgat gtcacatcca aaagctttcc agatgatctg
      841 cctcggatgc gattcaagtg cccgtactgc acacatgtgg tgaagcggaa ggcagaccta
      901 aagcgccacc ttcgttgtca tacaggagaa aggccctatc catgtcaagc ttgtggaaaa
      961 agatttagca ggctagacca tctaagtagc cattttcgaa caattcacca ggcatgtaaa
     1021 ctcatctgca gaaaatgtaa acgtcatgtg acagatctaa cagggcaagt ggtacaggag
     1081 ggaaccaggc gctacagact gtgtaatgag tgtcttgcag aatttggcat agacagcctc
     1141 cccattgact tggaagctga acaacatctt atgtccccat cagatggaga taaggattcc
     1201 agatggcact tgagtgaaga tgagaataga tcctatgtgg agattgtaga agatgggtct
     1261 gctgatctgg tcatccaaca ggttgatgat agtgaagaag aagaagaaaa agaaattaag
     1321 cccaacatta ggtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1381 tatatatctt gtggaaagga cgaccgaggg tatgacttcc aatacga
//
