LOCUS       sequence-280325-        1724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1099
FEATURES             Location/Qualifiers
     source          1..1724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcctgggt gctcaagatg gacgaggtga tcgagtccgg gctggtgcac
       61 gacttcgacg ccagcctctc gggcatcggg caggaactgg gcgccggcgc ttacagcatg
      121 agtgatgtct tggcattgcc cattttcaag caggaagatt ccagccttcc attggatggt
      181 gaaacagagc acccaccctt tcagtatgtg atgtgtgctg caacgtcacc agcagtaaaa
      241 ctgcatgatg aaacgcttac ttatttgaac caaggtcagt catatgaaat tcggatgctg
      301 gataatcgga aaatgggtga tatgcctgag atcaatggaa aattagtaaa gagcatcata
      361 agggttgtat tccatgacag acggctacaa tacacagagc atcagcaact tgaaggatgg
      421 aagtggaatc gcccaggaga cagacttctt gatttagata ttccaatgtc tgtgggaata
      481 attgacacaa ggacgaatcc aagccagtta aatgcggttg aatttctgtg ggacccagca
      541 aaacgcacct ctgctttcat tcaggtacac tgcatcagca cagaatttac tccacggaag
      601 cacggaggtg aaaagggagt gccctttagg atccaggttg acacctttaa gcagaatgaa
      661 aatggagaat acacagatca tctacactca gctagctgcc aaatcaaagt ttttaagcct
      721 aaaggtgcag acaggaaaca aaaaactgac cgagagaaga tggagaagag aacagctcat
      781 gaaaaagaaa agtatcagcc gtcctatgat accacaatcc tcacagagat gaggcttgag
      841 cctataattg aagatgcagt tgaacatgag cagaaaaagt ccagcaagcg gactttgcca
      901 gcagactacg gtgattctct ggcaaagcga ggcagttgtt ctccgtggcc cgatgccccc
      961 acagcctatg tgaataacag cccttcccca gcgcccactt tcacctcccc acagcagagc
     1021 acttgcagtg tcccagacag caattcttct tccccaaatc atcagggaga tggagcttca
     1081 cagacctctg gtgaacaaat tcagccttca gctacgatcc aggaaacaca gcaatggctg
     1141 ctcaaaaaca gattctcttc ctacacaaga ctgttctcta atttttcagg tgccgactta
     1201 ttaaaactga caaaggagga tttagttcaa atttgtggtg cagccgatgg aattcggctc
     1261 tataattcac tgaagtcaag gtcggttaga ccccgtttaa ccatctatgt ctgccgggag
     1321 cagccaagca gcacagtgct gcaagggcag cagcaagctg caagcagtgc aagcgagaat
     1381 ggcagtgggg caccctatgt ttatcatgca atctacttgg aagaaatgat tgcctcagaa
     1441 gttgctcgaa aacttgcgct ggtgtttaat atccctctcc accaaattaa tcaggtttac
     1501 agacagggtc ccaccggtat tcacattctt gttagtgatc agatggttca gaattttcaa
     1561 gatgagagtt gttttttatt ctccacagta aaagctgaaa gtagtgatgg catccacata
     1621 attttgaagt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1681 atatcttgtg gaaaggacga cgagacccta tggagcgtta agat
//
