LOCUS       sequence-280328-        3032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1103
FEATURES             Location/Qualifiers
     source          1..3032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccttgct gggtaagcgc tgtgacgtcc ccaccaacgg ctgcggaccc
       61 gaccgctgga actccgcgtt cacccgcaaa gacgagatca tcaccagcct cgtgtctgcc
      121 ttagactcca tgtgctcagc gctgtccaaa ctgaacgccg aggtggcctg tgtcgccgtg
      181 cacgatgaga gcgcctttgt ggtgggcaca gagaagggga gaatgttcct gaatgcccgg
      241 aaggagctac agtcagactt cctcaggttc tgcctctccg cagctcagca cagggcagcg
      301 acatcccagc tcgaaggccg ggtggtgaga cgggtgctca ctgtggcctc gcgtgctctg
      361 tgtcccacag gagggccccc gtggaaggat ccggaggcag agcaccccaa gaaggtgcag
      421 cggggcgagg gtggaggccg tagcctccct cggtcctccc tggaacatgg ctcagatgtg
      481 taccttctgc ggaagatggt agaggaggtg tttgatgttc tttatagcga ggccctggga
      541 agggccagtg tggtgccact gccctatgag aggctgctca gggagccagg gctgctggcc
      601 gtgcaggggc tgcccgaagg cctggccttc cgaaggccag ccgagtatga ccccaaggcc
      661 ctcatggcca tcctggaaca cagccaccgc atccgcttca agctcaagag gccacttgag
      721 gatggcgggc gggactcgaa ggccctggtg gagctgaacg gtgtctccct gattcccaag
      781 gggtcacggg actgtggcct gcatggccag gcccccaagg tgccacccca ggacctgccc
      841 ccaaccgcca cctcctcctc catggccagc ttcctgtaca gcacggcgct ccccaaccac
      901 gccatccgag agctcaagca ggaagcacct tcctgccccc ttgcccccag cgacctgggc
      961 ctgagtcggc ccatgccaga gcccaaggcc accggtgccc aagacttctc cgactgttgt
     1021 ggacagaagc ccactgggcc tggtgggcct ctcatccaga acgtccatgc ctccaagcgc
     1081 attctcttct ccatcgtcca tgacaagtca gagaagtggg acgccttcat aaaggaaacc
     1141 gaggacatca acacgctccg ggagtgtgtg cagatcctgt ttaacagcag atatgcggaa
     1201 gccctgggcc tggaccacat ggtccccgtg ccctaccgga agattgcctg tgacccggag
     1261 gctgtggaga tcgtgggcat cccggacaag atccccttca agcgcccctg cacttatgga
     1321 gtccccaagc tgaagcggat cctggaggag cgccatagta tccacttcat cattaagagg
     1381 atgtttgatg agcgaatttt cacagggaac aagtttacca aagacaccac gaagctggag
     1441 ccagccagcc cgccagagga cacctctgca gaggtctcta gggccaccgt ccttgacctt
     1501 gctgggaatg ctcggtcaga caagggcagc atgtctgaag actgtgggcc aggaacctcc
     1561 ggggagctgg gcgggctgag gccgatcaaa attgagccag aggatctgga catcattcag
     1621 gtcaccgtcc cagacccctc gccaacctct gaggaaatga cagactcgat gcctgggcac
     1681 ctgccatcgg aggattctgg ttatgggatg gagatgctga cagacaaagg tctgagtgag
     1741 gacgcgcggc ccgaggagag gcccgtggag gacagccacg gtgacgtgat ccggcccctg
     1801 cggaagcagg tggagctgct cttcaacaca cgatacgcca aggccattgg catctcggag
     1861 cccgtcaagg tgccgtactc caagtttctg atgcacccgg aggagctgtt tgtggtggga
     1921 ctgcctgaag gcatctccct ccgcaggccc aactgcttcg ggatcgccaa gctccggaag
     1981 attctggagg ccagcaacag catccagttt gtcatcaaga ggcccgagct gctcactgag
     2041 ggagtcaaag agcccatcat ggatagtcaa gagagggatt ccggggaccc tctggtggac
     2101 gagagcctga agagacaggg ctttcaagaa aattatgacg cgaggctctc acggatcgac
     2161 atcgccaaca cactaaggga gcaggtccag gaccttttca ataagaaata cggggaagcc
     2221 ttgggcatca agtacccggt ccaggtcccc tacaagcgga tcaagagtaa ccccggctcc
     2281 gtgatcatcg aggggctgcc cccaggaatc ccgttccgaa agccctgtac cttcggctcc
     2341 cagaacctgg agaggattct tgctgtggct gacaagatca agttcacagt caccaggcct
     2401 ttccaaggac tcatcccaaa gcctgatgaa gatgacgcca acagactcgg ggagaaggtg
     2461 atcctgcggg agcaggtgaa ggaactcttc aacgagaaat acggtgaggc cctgggcctg
     2521 aaccggccgg tgctggtccc ttataaacta atccgggaca gcccagacgc cgtggaggtc
     2581 acgggtctgc ctgatgacat ccccttccgg aaccccaaca cgtacgacat ccaccggctg
     2641 gagaagatcc tgaaggcccg agagcatgtc cgcatggtca tcattaacca gctccaaccc
     2701 tttgcagaaa tctgcaatga tgccaaggtg ccagccaaag acagcagcat tcccaagcgc
     2761 aagagaaagc gggtctcgga aggaaattcc gtctcctctt cctcctcgtc ttcctcttcc
     2821 tcgtcctcta acccggattc agtggcatcg gccaaccaga tctcactcgt gcaatggcca
     2881 atgtacatgg tggactatgc cggcctgaac gtgcagctcc cgggacctct taattactag
     2941 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     3001 aaggacgacc gcctagtagg cagagcctta at
//
