LOCUS       sequence-28033-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Chen H, Fre S, Slepnev VI, Capua MR, Takei K, Butler MH, Di Fiore 
            PP, De Camilli P
  TITLE     Epsin is an EH-domain-binding protein implicated in 
            clathrin-mediated endocytosis.
  JOURNAL   Nature. 1998 Aug 20. 394(6695):793-7.
  PUBMED    9723620
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1998 Aug 20. 394(6695):793-7."
COMMENT     Sequence Label: pCDNA3.1-EPSIN 1
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             130..147
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             151..183
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(187..195,196..210)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 gggggcatta gcgagctctc tggctactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttaccatg
      121 gggggttctc atcatcatca tcatcatggt atggctagca tgactggtgg acagcaaatg
      181 ggtcgggatc tgtacgacga tgacgataag gtacctaagg atccagtgtg gtggaattct
      241 gcagatatcc agcacagtgg cggccgctcg acatcatcgc tgcggcgtca gatgaagaat
      301 atcgtccaca actactcaga ggcagagatc aaggtccggg aggccacaag caatgatccc
      361 tggggcccat ccagctccct catgtcagag attgctgacc tcacctacaa tgttgtggcc
      421 ttctccgaga ttatgagcat gatctggaag cggctcaacg accatggcaa gaattggagg
      481 cacgtctaca aggccatgac gttgatggag tacctcatca agactggctc tgagcgtgta
      541 tcacaacagt gcaaagagaa catgtatgct gtgcagacgc tgaaggactt ccaatatgtg
      601 gaccgtgatg gcaaggacca gggtgtgaat gtgcgggaga aggccaagca gctggtggct
      661 ttgctgcgtg atgaggatcg gcttcgggag gagcgcgccc acgcgctcaa gaccaaggag
      721 aaactggcac agactgccac ggcttcctca gcagctgtgg gctctggacc acctcccgag
      781 gcggagcagg catggccaca gagcagcggg gaggangagt tgcagctcca gctggccctg
      841 gctatgagca aggaggaggc tgaccagccc ccgtcctgcg gccccgagga tgacgtccag
      901 ctccagctgg ccctnagttt gagccgagag gncccgataa ggaagancgg atccgtcntg
      961 gggatgacct ga
//
