LOCUS       sequence-280334-        2291 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1121
FEATURES             Location/Qualifiers
     source          1..2291
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggaaaaaa atacaagaac attgttctac taaaaggatt agaggtcatc
       61 aatgattatc attttagaat ggttaagtcc ttactgagca acgatttaaa acttaattta
      121 aaaatgagag aagagtatga caaaattcag attgctgact tgatggaaga aaagttccga
      181 ggtgatgctg gtttgggcaa actaataaaa attttcgaag atataccaac gcttgaagac
      241 ctggctgaaa ctcttaaaaa agaaaagtta aaagtaaaag gaccagccct atcaagaaag
      301 aggaagaagg aagtggatgc tacttcacct gcaccctcca caagcagcac tgtcaaaact
      361 gaaggagcag aggcaactcc tggagctcag aaaagaaaaa aatcaaccaa agaaaaggct
      421 ggacccaaag ggagtaaggt gtccgaggaa cagactcagc ctccctctcc tgcaggagcc
      481 ggcatgtcca cagccatggg ccgttcccca tctcccaaga cctcattgtc agctccaccc
      541 aacagttctt caactgagaa cccgaaaaca gtggccaaat gtcaggtaac tcccagaaga
      601 aatgttctcc aaaaacgccc agtgatagtg aaggtactga gtacaacaaa gccatttgaa
      661 tatgagaccc cagaaatgga gaaaaaaata atgtttcatg ctacagtggc tacacagaca
      721 cagttcttcc atgtgaaggt tttaaacacc agcttgaagg agaaattcaa tggaaagaaa
      781 atcatcatca tatcagatta tttggaatat gatagtctcc tagaggtcaa tgaagaatct
      841 actgtatctg aagctggtcc taaccaaacg tttgaggttc caaataaaat catcaacaga
      901 gcaaaggaaa ctctgaagat tgatattctt cacaaacaag cttcaggaaa tattgtatat
      961 ggggtattta tgctacataa gaaaacagta aatcagaaga ccacaatcta cgaaattcag
     1021 gatgatagag gaaaaatgga tgtagtgggg acaggacaat gtcacaatat cccctgtgaa
     1081 gaaggagata agctccaact tttctgcttt cgacttagaa aaaagaacca gatgtcaaaa
     1141 ctgatttcag aaatgcatag ttttatccag ataaagaaaa aaacaaaccc gagaaacaat
     1201 gaccccaaga gcatgaagct accccaggaa cagcgtcagc ttccatatcc ttcagaggcc
     1261 agcacaacct tccctgagag ccatcttcgg actcctcaga tgccaccaac aactccatcc
     1321 agcagtttct tcaccaagaa aagtgaagac acaatctcca aaatgaatga cttcatgagg
     1381 atgcagatac tgaaggaagg gagtcatttt ccaggaccgt tcatgaccag cataggccca
     1441 gctgagagcc atccccacac tcctcagatg cctccatcaa caccaagcag cagtttctta
     1501 accacgttga aaccaagact gaagactgaa cctgaagaag tttccataga agacagtgcc
     1561 cagagtgacc tcaaagaagt gatggtgctg aacgcaacag aatcatttgt atatgagccc
     1621 aaagagcaga agaaaatgtt tcatgccaca gtggcaactg agaatgaagt cttccgagtg
     1681 aaggttttta atattgacct aaaggagaag ttcaccccaa agaagatcat tgccatagca
     1741 aattatgttt gccgcaatgg gttcctggag gtatatcctt tcacacttgt ggctgatgtg
     1801 aatgctgacc gaaacatgga gatcccaaaa ggattgatta gaagtgccag cgtaactcct
     1861 aaaatcaatc agctttgctc acaaactaaa ggaagttttg tgaatggggt gtttgaggta
     1921 cataagaaaa atgtaagggg tgaattcact tattatgaaa tacaagataa tacagggaag
     1981 atggaagtgg tggtgcatgg acgactgacc acaatcaact gtgaggaagg agataaactg
     2041 aaactcacct gctttgaatt ggcaccgaaa agtgggaata ccggggagtt gagatctgta
     2101 attcatagtc acatcaaggt catcaagacc aggaaaaaca agaaagacat actcaatcct
     2161 gattcaagta tggaaacttc accagacttt ttcttctagt aatgaactag tccgtaactt
     2221 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagcc atgcatgacc
     2281 agcacgaact t
//
