LOCUS       sequence-280340-        2279 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1133
FEATURES             Location/Qualifiers
     source          1..2279
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaacaatc taatgattca ttaagagtca accataatga cggtgaagag
       61 tcaaaaacca gtgctcaagt atttgagcat ctaatctgta tggactccag ggattcttcc
      121 tttggacaaa atgattctcc tacagttttg cccatcacta ctcgtgaagc aaataattca
      181 ctcatatcac agaatatacc agggcccctg actcagacac agactctttc tgcagagcaa
      241 ttccatctag tggaccaaaa tgggcaggct attcaatatg aacttcagtc attgggggaa
      301 tccaatgcac aaatgatgat cgttgccagc ccaacagaaa atggacaggt acttcgtgta
      361 attccaccta cccagacagg aatggcacaa gtgattatac ctcaggggca acttgtggat
      421 gtgaatagtc ctcgggatgt ccctgaagag aaacccagta acagaaactt accaactgta
      481 agagtggata ctctagcaga caataccagc aattacattc ttcatcctca aacatccttc
      541 ccattgccca aaaagtcagt gaccggaatg ctggaagaac cccttctggg gcctcttcag
      601 ccactttctt ctaatacacc tatatgggcc tgccgtctta ggagctgtga gaaaattgga
      661 gattcatacc gtggctactg tgtaagtgag actgaattag aaagtgtcct aacatttcac
      721 aagcagcaaa cacagagtgt ttgggggacc cgtcagtctc caagcccagc caagcctgct
      781 acacgcttga tgtggaaatc ccagtatgtt ccatatgatg gaatcccatt tgttaatgca
      841 gggagtagag ctgtggtaat ggagtgtcag tatgggccaa gaagaaaagg tttccagtta
      901 aaaaaagtca gtgagcagga aagcaggtct tgtcagctct acaaagccac ttgtccagct
      961 cggatttaca ttaaaaaggt acagaagttt cctgaatata gagttcctac agaccccaaa
     1021 attgacaaga aaattatcag aatggagcag gagaaagctt ttaacatgct aaagaagaac
     1081 ttggtagatg ctggtggtgt tcttaggtgg tatgtacagt tacctacaca gcaagctcat
     1141 cagtatcatg aattagagac tccctgcctc actttgtcac cttctccttt tcctgtgtct
     1201 tctcttgaag aagaggaaac tgcagttaga gatgagaatt gtgcattacc ctcacgttta
     1261 catcctcaag tagcacataa gattcaagaa ttagtatcac agggaataga acaagtgtat
     1321 gcagtaagga aacagctaag aaaatttgtg gaaagggaac tgttcaaacc cgatgaggta
     1381 cctgaaagac ataatttatc tttttttcca actgtaaatg atataaaaaa tcacatccat
     1441 gaggtacaga aatccttgag aaatggagat acggtatata actcagagat tattccagca
     1501 acgcttcaat ggactacaga cagtgggaat attctcaaag agaccatgac agttacattt
     1561 gcagaaggaa attcaccagg agaatcaatt accaccaaag tggaaacaaa tcagaccagg
     1621 ggttctttgt ctcctgagcc aacccacttg ctctcctcac tctcctcatt tcagcccaaa
     1681 atatttacac aactacaggg tttgcagtta caaccaaggt acacctctcc tgatgaatca
     1741 ccagctgtgg tatcagtaaa taaccagccg tcctctagtc cttcaggact tctggataca
     1801 ataggaagtg ctgtaatgaa taataattct ctactgcttg gtcaaagtca tagccttcaa
     1861 agagatacat gcttaaccca aaacaatagt actgcctcca ccatgggtaa ccttccagaa
     1921 ccagatcaaa atctagttgc aatggacgag ctggtagaag ttggagatgt tgaggataca
     1981 gggaatctgg aaggaactgt tcatcggatt ctgttgggag atgtgcagac tattccaata
     2041 cagattatag acaaccactc agctcttatt gaagaaaatc cagaaagtac catttctgtg
     2101 agccaagtta aacaagaacc caaagaacca gcattgtcta tggaagcaaa aaaaactgtg
     2161 gactataaga aattatctgc tacatagtaa tgaactagtc cgtaacttga aagtatttcg
     2221 atttcttggc tttatatatc ttgtggaaag gacgaactct accactccat tccactgcc
//
