LOCUS       sequence-280358-        1178 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1161
FEATURES             Location/Qualifiers
     source          1..1178
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcctccac ttcccgccgc caacgccgag aacgtcgctt tcgtcgttac
       61 ttgtctgcag gacggctggt ccgggcccag gccctcctcc agcgacaccc aggcctcgat
      121 gtagatgctg ggcagccccc accactgcac cgggcctgtg cccgccacga tgcccctgcc
      181 ctgtgcctgc tgcttcggct cggggctgac cctgcccacc aggaccgcca tggggacacg
      241 gcactgcatg ctgctgcccg ccagggccca gatgcctaca ccgatttctt cctcccgctg
      301 ctaagccgct gtccctccgc catgggaata aagaataagg atggggagac ccctggccaa
      361 attttgggct ggggaccccc ctgggattct gctgaagagg aggaagaaga tgatgcctcc
      421 aaggagcggg aatggagaca gaagctccag ggtgagctgg aggacgagtg gcaggaagtc
      481 atggggaggt ttgaaggtga tgcctcccat gaaacccagg aacctgagtc cttctcagcc
      541 tggtcagatc gcctggcccg ggaacatgcc cagaagtgcc agcagcagca gcgagaagca
      601 gagggatccc gtcgaccccc acgtgctgag ggctccagcc agagctggcg acagcaggag
      661 gaggagcagc ggctcttcag ggagcgagcc cgggccaagg aggaagagct gcgtgagagc
      721 cgagccagga gggcgcagga ggctctaggg gaccgagaac ccaagccaac cagggccggg
      781 cccagggaag agcaccccag aggagcgggg aggggcagcc tctggcgatt tggtgatgtg
      841 ccctggccct gccctggggg aggggaccca gaggccatgg ctgcagccct ggtggccagg
      901 ggcccccctt tggaggaaca gggggctctg aggaggtact tgagggtcca gcaggtccgc
      961 tggcaccctg accgcttcct gcagcgattc cgaagccaga ttgagacctg ggagctgggc
     1021 cgtgtgatgg gagcagtgac agccctttct caggccctga atcgccatgc agaggccctc
     1081 aagtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1141 tgtggaaagg acgagtacga tttcccgcct tagctaag
//
