LOCUS       sequence-280367-        2132 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1165
FEATURES             Location/Qualifiers
     source          1..2132
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgccgtt cctgcgcatc gccttcaact cctatgagct gggctccctg
       61 caggccgagg acgaggcgaa ccagcccttc tgtgccgtga agatgaagga ggcgctcagc
      121 acagagcgtg ggaaaacact ggtgcagaag aagccgacca tgtatcctga gtggaagtcg
      181 acgttcgatg cccacatcta tgaggggcgc gtcatccaga ttgtgctaat gcgggcagca
      241 gaggagccag tgtctgaggt gaccgtgggt gtgtcggtgc tggccgagcg ctgcaagaag
      301 aacaatggca aggctgagtt ctggctggac ctgcagcctc aggccaaggt gttgatgtct
      361 gttcagtatt tcctggagga cgtggattgc aaacagtcta tgcgcagtga ggacgaggcc
      421 aagttcccaa cgatgaaccg ccgcggagcc atcaaacagg ccaaaatcca ctacatcaag
      481 aaccatgagt ttatcgccac cttctttggg caacccacct tctgttctgt gtgcaaagac
      541 tttgtctggg gcctcaacaa gcaaggctac aaatgcaggc aatgtaacgc tgccatccac
      601 aagaaatgca tcgacaagat catcggcaga tgcactggca ccgcggccaa cagccgggac
      661 actatattcc agaaagaacg cttcaacatc gacatgccgc accgcttcaa ggttcacaac
      721 tacatgagcc ccaccttctg tgaccactgc ggcagcctgc tctggggact ggtgaagcag
      781 ggattaaagt gtgaagactg cggcatgaat gtgcaccata aatgccggga gaaggtggcc
      841 aacctctgcg gcatcaacca gaagcttttg gctgaggcct tgaaccaagt cacccagaga
      901 gcctcccgga gatcagactc agcctcctca gagcctgttg ggatatatca gggtttcgag
      961 aagaagaccg gagttgctgg ggaggacatg caagacaaca gtgggaccta cggcaagatc
     1021 tgggagggca gcagcaagtg caacatcaac aacttcatct tccacaaggt cctgggcaaa
     1081 ggcagcttcg ggaaggtgct gcttggagag ctgaagggca gaggagagta ctttgccatc
     1141 aaggccctca agaaggatgt ggtcctgatc gacgacgacg tggagtgcac catggttgag
     1201 aagcgggtgc tgacacttgc cgcagagaat ccctttctca cccacctcat ctgcaccttc
     1261 cagaccaagg accacctgtt ctttgtgatg gagttcctca acggggggga cctgatgtac
     1321 cacatccagg acaaaggccg ctttgaactc taccgtgcca cgttttatgc cgctgagata
     1381 atgtgtggac tgcagtttct acacagcaag ggcatcattt acagggacct caaactggac
     1441 aatgtgctgc tggaccggga tggccacatc aagattgccg actttgggat gtgcaaagag
     1501 aacatattcg gggagagccg ggccagcacc ttctgcggca cccctgacta tatcgcccct
     1561 gagatcctac agggcctgaa gtacacattc tctgtggact ggtggtcttt cggggtcctt
     1621 ctgtacgaga tgctcattgg ccagtccccc ttccatggtg atgatgagga tgaactcttc
     1681 gagtccatcc gtgtggacac gccacattat ccccgctgga tcaccaagga gtccaaggac
     1741 atcctggaga agctctttga aagggaacca accaagaggc tgggagtgac cggaaacatc
     1801 aaaatccacc ccttcttcaa gaccataaac tggactctgc tggaaaagcg gaggttggag
     1861 ccacctttca ggcccaaagt gaagtcaccc agagactaca gtaactttga ccaggagttc
     1921 ctgaacgaga aggcgcgcct ctcctacagc gacaagaacc tcatcgactc catggaccag
     1981 tctgcattcg ctggcttctc ctttgtgaac cccaaattcg agcacctcct ggaagattag
     2041 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     2101 aaggacgacg tgattgcttc ttggcgcacc ga
//
