LOCUS       sequence-28038-e        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Salazar MA, Kwiatkowski AV, Pellegrini L, Cestra G, Butler MH, 
            Rossman KL, Serna DM, Sondek J, Gertler FB, De Camilli P
  TITLE     Tuba, a novel protein containing bin/amphiphysin/Rvs and Dbl 
            homology domains, links dynamin to regulation of the actin 
            cytoskeleton.
  JOURNAL   J Biol Chem. 2003 Dec 5. 278(49):49031-43.
  PUBMED    14506234
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Dec 5. 278(49):49031-43."
COMMENT     Sequence Label: pcDNA3-HA-Tuba
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             106..132
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gggngcatta gcagagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa
       61 ttaatacgac tcactatagg gagacccaag cttggtacca tggcgtaccc atacgacgtc
      121 ccagactacg cgggatccga attgatggag gctggctcag tggttcgagc catttttgac
      181 ttctgcccta gcgtatcaga agaactgccg ctctttgtgg gagatattat tgaggtgctg
      241 gcagtggtgg atgaattttg gcttctagga aagaaggagg atgttacagg acaattcccc
      301 agcagttttg tggaaattgt gaccattccc agtctaaaag agggagagag gctgtttgtg
      361 tgcatttgtg aattcacatc ccaagagttg gataatcttc ccctccatcg aggtgacctg
      421 gtgattctcg atggcattcc cactgcaggc tggctgcagg gccgaagctg ctggggcgca
      481 cggggcttct tcccatcttc atgtgtccgc gagctctgcc tctcctcaca gagccggcag
      541 tggcactccc agagcgccct gtttcagatt ccggaatatt ccatgggaca agcccgggcc
      601 ctaatggggc tttctgctca gctggatgaa gagctggact tcagggaagg ggatgtgatc
      661 accattattg gagttcctga accaggctgg tttgaagggg agttagaggg ccgaagaggc
      721 atttttccag aaggttttgt agagctgttg gggcccctga ggactgtgga tgagtcagta
      781 agttctggaa atcaagatga ctgcattgtt taatggtgaa gtagataccc cccgtaggag
      841 aagaagagat agggcccgga tgaggatgag gagganccag ggacctatgg gggtcgccct
      901 gtacagaatn ccaagccctg gagccaaatg aacctggatt tcgagggnng ggaaaaaant
      961 ccgaattctg gcgaaccttg gaaaaaatgg ctggctgaaa ggaancc
//
