LOCUS       sequence-280394-        2261 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1203
FEATURES             Location/Qualifiers
     source          1..2261
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggagaaaa aaatggcgat gcaaaaactt tctggatgga gctagaagat
       61 gatggaaaag tggacttcat ttttgaacaa gtacaaaatg tgctgcagtc actgaaacaa
      121 aagatcaaag atgggtctgc caccaataaa gaatacatcc aagcaatgat tctagtgaat
      181 gaagcaacta taattaacag ttcaacatca ataaagggag catcacagaa agaagtgaat
      241 gcccaaagca gtgatcctat gcctgtgact cagaaggaac aggaaaacaa atccaatgca
      301 tttccctcta catcatgtga aaactccttt ccagaagact gtacatttct aacaacagaa
      361 aataaggaaa ttctctctct tgaagataaa gttgtagact ttagagaaaa agactcatct
      421 tcgaatttat cttaccaaag tcatgactgc tctggtgctt gtctgatgaa aatgccactg
      481 aacttgaagg gagaaaaccc tctgcagctg ccaatcaaat gtcacttcca aagacgacat
      541 gcaaagacaa actctcattc ttcagcactc cacgtgagtt ataaaacccc ttgtggaagg
      601 agtctacgaa acgtggagga agtttttcgt tacctgcttg agacagagtg taacttttta
      661 tttacagata acttttcttt caatacctat gttcagttgg ctcggaatta cccaaagcaa
      721 aaagaagttg tttctgatgt ggatattagc aatggagtgg aatcagtgcc catttctttc
      781 tgtaatgaaa ttgacagtag aaagctccca cagtttaagt acagaaagac tgtgtggcct
      841 cgagcatata atctaaccaa cttttccagc atgtttactg attcctgtga ctgctctgag
      901 ggctgcatag acataacaaa atgtgcatgt cttcaactga cagcaaggaa tgccaaaact
      961 tcccccttgt caagtgacaa aataaccact ggatataaat ataaaagact acagagacag
     1021 attcctactg gcatttatga atgcagcctt ttgtgcaaat gtaatcgaca attgtgtcaa
     1081 aaccgagttg tccaacatgg tcctcaagtg aggttacagg tgttcaaaac tgagcagaag
     1141 ggatggggtg tacgctgtct agatgacatt gacagaggga catttgtttg catttattca
     1201 ggaagattac taagcagagc taacactgaa aaatcttatg gtattgatga aaacgggaga
     1261 gatgagaata ctatgaaaaa tatattttca aaaaagagga aattagaagt tgcatgttca
     1321 gattgtgaag ttgaagttct cccattagga ttggaaacac atcctagaac tgctaaaact
     1381 gagaaatgtc caccaaagtt cagtaataat cccaaggagc ttactgtgga aacgaaatat
     1441 gataatattt caagaattca atatcattca gttattagag atcctgaatc caagacagcc
     1501 atttttcaac acaatgggaa aaaaatggaa tttgtttcct cggagtctgt cactccagaa
     1561 gataatgatg gatttaaacc accccgagag catctgaact ctaaaaccaa gggagcacaa
     1621 aaggactcaa gttcaaacca tgttgatgag tttgaagata atctgctgat tgaatcagat
     1681 gtgatagata taactaaata tagagaagaa actccaccaa ggagcagatg taaccaggcg
     1741 accacattgg ataatcagaa tattaaaaag gcaattgagg ttcaaattca gaaaccccaa
     1801 gagggacgat ctacagcatg tcaaagacag caggtatttt gtgatgaaga gttgctaagt
     1861 gaaaccaaga atacttcatc tgattctcta acaaagttca ataaagggaa tgtgttttta
     1921 ttggatgcca caaaagaagg aaatgtcggc cgcttcctta atcatagttg ttgcccaaat
     1981 ctcttggtac agaatgtttt tgtagaaaca cacaacagga attttccatt ggtggcattc
     2041 ttcaccaaca ggtatgtgaa agcaagaaca gagctaacat gggattatgg ctatgaagct
     2101 gggactgtgc ctgagaagga aatcttctgc caatgtgggg ttaataaatg tagaaaaaaa
     2161 atattatagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     2221 tcttgtggaa aggacgaagt ggagacgtta tgcaaggtgt a
//
