LOCUS       sequence-280397-        2048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1206
FEATURES             Location/Qualifiers
     source          1..2048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1230..1241)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tggctacagc cgtggaacca gaggaccagg atctttggga agaagaggga
       61 attctgatgg tgaaactgga agatgatttc acctgtcggc cagagtctgt cttacagagg
      121 gatgacccgg tgctggaaac ctcccaccag aacttccgac gcttccgcta ccaggaggca
      181 gcaagcccta gagaagctct catcagactc cgagaacttt gtcaccagtg gctgagacca
      241 gagaggcgga caaaggagca gatcctagag ctgcttgtgc tggaacaatt tcttaccgtc
      301 ctacctggag aactacagag ctgggtgcgg ggccaacggc cagaaagtgg cgaggaggca
      361 gtgacgctgg tggagggttt gcagaaacaa cccaggagac caaggcggtg ggtgactgtc
      421 catgttcacg gccaggaagt cctgtcagag gagacggtgc atttaggagt ggagcctgag
      481 tcacctaatg agctgcagga tcctgtgcaa agctcgaccc ccgagcagtc tcctgaggaa
      541 accacacaga gcccagatct gggggcaccg gcagagcagc gtccacacca ggaagaggag
      601 ctccagaccc tgcaggagag cgaggtccca gtgcccgagg acccagacct tcctgcagag
      661 aggagctctg gagactcaga gatggttgct cttcttactg ctctgtcaca gggactggta
      721 acgttcaagg atgtggccgt atgcttttcc caggaccagt ggagtgatct ggacccaaca
      781 cagaaagagt tctatggaga atatgtcttg gaagaagact gtggaattgt tgtctctctg
      841 tcatttccaa tccccagacc tgatgagatc tcccaggtta gagaggaaga gccttgggtc
      901 ccagatatcc aagagcctca ggagactcaa gagccagaaa tcctgagttt tacctacaca
      961 ggagatagga gtaaagatga ggaagagtgt ctggagcagg aagatctgag tttggaggat
     1021 atacacaggc ctgttttggg agaaccagaa attcaccaga ctccagattg ggaaatagtc
     1081 tttgaggaca atccaggtag acttaatgaa agaagatttg gtactaatat ttctcaagtg
     1141 aatagttttg tgaaccttcg ggaaactaca cccgtccacc ccctgttagg gaggcatcat
     1201 gactgttctg tgtgtggaaa gagcttcact tgtaactccc accttgttag acacctgagg
     1261 actcacacag gagagaaacc ctataaatgt atggaatgtg gaaaaagtta cacacgaagc
     1321 tcacatcttg ccaggcacca aaaggttcac aagatgaacg cgccttacaa atatccccta
     1381 aaccggaaga atttggaaga gacctcccct gtgacacagg ctgagagaac tccatcagtg
     1441 gagaaaccct atagatgtga tgattgcgga aagcacttcc gctggacttc agaccttgtc
     1501 agacatcaga ggacacatac tggagaaaaa cccttctttt gtactatttg tggcaaaagc
     1561 ttcagccaga aatctgtgtt aacaacacac caaagaatcc acctgggagg caaaccctac
     1621 ttgtgtggag agtgtggtga ggacttcagt gaacacaggc ggtacctggc gcaccggaag
     1681 acgcacgctg ctgaggaact ctacctctgc agcgagtgcg ggcgctgctt cacccacagc
     1741 gcagcgttcg ccaagcactt gagaggacac gcctcagtga ggccctgccg atgcaacgaa
     1801 tgtgggaaga gcttcagtcg cagggaccac ctcgtcaggc atcagagaac acacactggg
     1861 gagaaaccat tcacgtgccc tacctgtgga aaaagcttca gcagaggata tcacttaatt
     1921 aggcatcaga ggacccactc agaaaagacc tcctagtaat gaactagtcc gtaacttgaa
     1981 agtatttcga tttcttggct ttatatatct tgtggaaagg acgataaatg gtacgcggtt
     2041 gggatggc
//
